Temporal dynamics of pathogenesis-related metabolites and their plausible pathways of induction in potato leaves following inoculation with <Emphasis Type="Italic">Phytophthora infestans</Emphasis> |
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Authors: | Y Abu-Nada A C Kushalappa W D Marshall K Al-Mughrabi A Murphy |
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Institution: | (1) Department of Plant Science, McGill University, Ste. Anne de Bellevue, Montreal, QC, H9X 3V9, Canada;(2) Department of Food Science and Agriculture Chemistry, McGill University, Ste. Anne de Bellevue, Montreal , QC, H9X 3V9, Canada;(3) New Brunswick Department of Agriculture, Wicklow, NB, E7L 3S4, Canada;(4) Agriculture and Agri-Food Canada, Fredericton, NB, E3B 4Z7, Canada |
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Abstract: | Metabolite profiles based on GC/MS were used to study the temporal dynamics of metabolites in potato leaves following pathogen
inoculation. In the polar and non-polar plant extracts a total of 106 consistent peaks were detected, of which 95 metabolites
were tentatively identified. Following pathogen inoculation, the abundances of 42 metabolites were significantly increased
or decreased, and these metabolites were designated as Pathogenesis-Related (PR) Metabolites. Factor analysis of the abundance
of 106 metabolites identified four plant–pathogen interaction functions: (i) homeostasis; (ii) primary defence; (iii) secondary
defence; (iv) collapse of primary and secondary defence responses. During the primary and secondary defence phases, dramatic
changes in the amino acids, known precursors of several plant defence-related metabolites, were observed. Plausible satellite-networks
of metabolic pathways leading to the up-regulation of these families of amino acids and other secondary metabolites, and their
potential application for the evaluation of horizontal resistance in potato against the late blight pathogen is discussed. |
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Keywords: | FACTOR Analysis Horizontal resistance GC/MS Lee Retention Index Metabolomics PR-metabolites Solanum tuberosum |
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