中国明对虾单个家系中与生长性状相关微卫星标记的初步筛选

以1个人工授精获得的中国明对虾(Fenneropenaeus chinensis)F2家系为材料,对其中80尾中国明对虾进行正态分布检验,选择19个稳定扩增的微卫星位点对每一个体进行扩增和基因分型,分析家系遗传信息.利用最小二乘法对微卫星位点与中国明对虾体长、全长、体质量性状进行关联性分析.采用SPSS 11.5软件作微卫星分子标记与经济性状的方差分析,考察各微卫星位点对体长、全长、体质量3个经济性状的影响程度.结果表明,中国明对虾体长、全长、体质量3个性状均呈正态分布(P＞0.05);相关分析得到,19个微卫星位点中RS0683和FC019两个微卫星位点与体长、全长、体质量呈显著相关(P＜0.05);同时对差异显著的位点进行不同基因型间经济性状的多重比较,RS0683标记座位AA基因型在体长、全长、体质量的表型效应上极显著高于AB、BB基因型(P＜0.01),表明该标记位点基因型AA与3种经济性状正相关;FC019标记座位的AA基因型体长、全长、体质量显著低于AB、BC基因型(P＜0.05),表明该标记位点基因型AA与3种经济性状呈负相关.

Screening of microsatellite DNA markers associated with growth traits in a single Fenneropenaeus chinensis family

 Microsatellite DNA markers are increasingly being used to improve selective breeding programs. Currently, there is a need for molecular markers associated with traits for marker assisted selection in shrimp breeding programs. We analyzed the association between microsatellite DNA markers and body length, total body height, and body weight in an F2 family of Chinese shrimp (Fenneropenaeus chinensis) that were mated by artificial insemination. Eighty individuals from the F2 family were chosen for the normal distribution test. Nineteen microsatellite primer pairs were obtained to amplify the total DNA and genotype each locus. We calculated the mean number of alleles, mean expected heterozygosity, mean polymorphic information content. Genotypic differences of these microsatellites loci between the maximal high group and minimal low group were analyzed using SPSS11.5 software. One-way ANOVA was conducted to test the association between the individual marker alleles and the three economic traits. The body length, total body height, and body weight were normally distributed(P>0.05). The mean number of alleles, mean expected heterozygosity, and mean polymorphic information content were 2.470, 0.533 and 0.442, respectively. The microsatellite loci RS0683 and FC019 were significantly associated with body weight, body length, and total body height (P<0.05 or P<0.01). The most favorable genotypes for growth traits at the microsatellite locus RS0683 was AA (P<0.01) and the most unfavorable at locus FC019 was AA (P<0.05). We speculate that the genotype AA was positively associated with the three economic traits at locus RS0683 and the genotype AA was negatively associated with the traits at locus FC019. Our results suggest that selective genotyping integrates the advantages of BSA and marker-based analysis to improve our ability to detect an association between quantitative trait locus and markers. Two of the microsatellite loci had a significant effect on the three economic traits. Identification of a large number of DNA markers linked to QTL controlling the resistance to WSSV will contribute to the application of DNA marker-assisted selection during breeding of Chinese shrimp for intensive culture