| 微波消解-恒pH滴定法快速测定粮食中的粗蛋白 |
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| 引用本文： | 高向阳,黄进慧,张震芳,付娜. 微波消解-恒pH滴定法快速测定粮食中的粗蛋白[J]. 粮食储藏, 2008, 37(5) |
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| 作者姓名： | 高向阳 黄进慧 张震芳 付娜 |
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| 作者单位： | 河南农业大学食品科学技术学院,郑州,450002;河南农业大学食品科学技术学院,郑州,450002;河南农业大学食品科学技术学院,郑州,450002;河南农业大学食品科学技术学院,郑州,450002 |
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| 基金项目： | 河南省教育厅自然科学基金 |
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| 摘 要： | 为建立一种快速测定食品中粗蛋白质的新型分析方法,选择小麦为样品,用硫酸一过氧化氢混合溶液为消解剂,利用微波压力程序加热技术快速消解样品,用甲醛法将NH4 转化为H ,以KOH为标准溶液,用恒pH滴定法进行测定.结果表明:小麦干基粗蛋白的含量在14%～17%之间,RSD1.2%～2.1%(n=6或12).与经典方法对照测定,经F检验和t检验表明两者之间无显著性差异(置信度95%).该法操作简单、快速,试剂用量少,空白值低,工作效率大大提高,结果令人满意.
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| 关 键 词： | 微波消解 恒pH滴定法 粗蛋白质 小麦 |
RAPID DETERMINATION OF CRUDE PROTEIN IN GRAIN WITH CONSTANT pH METHOD BY MICROWAVE DIGESTION |
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| Gao Xiangyang,Huang Jinhui,Zhang Zhenfang,Fu Na. RAPID DETERMINATION OF CRUDE PROTEIN IN GRAIN WITH CONSTANT pH METHOD BY MICROWAVE DIGESTION[J]. www.ysfri.ac.cn, 2008, 37(5) |
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| Authors: | Gao Xiangyang Huang Jinhui Zhang Zhenfang Fu Na |
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| Affiliation: | Gao Xiangyang Huang Jinhui Zhang Zhenfang Fu Na(College of Food Science , Technology,Henan Agricultural University,Zhengzhou 450002) |
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| Abstract: | In order to establish a rapid analysis method of determination crude protein in food,we selected wheat as samples,which were digested with sulfuric acid-hydrogen peroxide mixture solution,using the microwave pressure procedure heating technology for rapid digestion samples,using formaldehyde method which changed NH4+ into H+,and KOH as the standard solution,wheat samples were determinated by constant pH titration method.The results indicated that the content of crude protein in dry wheat is 14-17%,the relat... |
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| Keywords: | microwave digestion constant pH titration method protein wheat |
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