| 抗鸡败血支原体单克隆抗体杂交瘤细胞株的建立 |
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| 引用本文： | 杨百亮 廉慧锋. 抗鸡败血支原体单克隆抗体杂交瘤细胞株的建立[J]. 中国预防兽医学报, 1999, 21(3): 199-201 |
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| 作者姓名： | 杨百亮 廉慧锋 |
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| 作者单位： | 天津农学院动科系,山西农业大学动医系 |
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| 基金项目： | 天津市教委科技计划项目 |
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| 摘 要： | 应用杂交瘤技术获得了４株抗鸡败血支原体单克隆抗体。４株单克隆抗体与鸡滑液支原体、传染性支气管炎病毒、鸡新城疫病毒、鸡白痢沙门氏菌和大肠杆菌抗原均无交叉反应。１株单克隆抗体为ＩｇＧ２ａ,其余３株单克隆抗体均为ＩｇＭ。４株杂交瘤细胞染色体均有少量中部或亚中部着丝点染色体。
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| 关 键 词： | 鸡败血支原体 单克隆抗体 |
TheAcquirementofHybridomasSecretingMonoclonal AntibodiesSpecifictoMycoplasmagallisepticum |
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| Author YANGBailiangLIANHuifeng,GUOJianhua,ZHAOCuiping. TheAcquirementofHybridomasSecretingMonoclonal AntibodiesSpecifictoMycoplasmagallisepticum[J]. Chinese Journal of Preventive Veterinary Medicine, 1999, 21(3): 199-201 |
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| Authors: | Author YANGBailiangLIANHuifeng GUOJianhua ZHAOCuiping |
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| Affiliation: | Author YANGBailiang1LIANHuifeng2,GUOJianhua2,ZHAOCuiping1 |
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| Abstract: | Four monoclonalantibodies(McAbs)wereproducedagainstS6strainofMycoplasmagallisepticumusinghybridomatechnique.No crossreactionwasobservedbetweentheMcAbsandNewcastlediseasevirus,infectiousbronchitisvirus, E.coli, S.pullorumandmycoplasmasynoviae.TheMcAbsecretedbyhybridomacell1C5wasclassifiedasIgG2a.However,theotherthreeonessecretedonlythetypeofIgMimmunoglobulin.Theresultsofidentifyingthechromosomesshowedthatthechromosomesofallthefourstrainsofhybridomacellshaveafewofcentroneresorsubcentromeres. |
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| Keywords: | Mycoplasmagallisepticum Monoclonalantibodies |
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