| 抗bFGF鼠单克隆抗体轻链可变区基因的克隆及序列分析 |
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| 引用本文： | 王德文等,刘纯杰.抗bFGF鼠单克隆抗体轻链可变区基因的克隆及序列分析[J].中国预防兽医学报,1999,21(5):359-362. |
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| 作者姓名： | 王德文等 刘纯杰 |
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| 作者单位： | 解放军农牧大学动物医学系!长春130062(刘纯杰，郑兆荣)，军事医学科学院放射医学研究所!北京100850(王德文，高亚兵，熊呈琦)，军事医学科学院生物工程研究所!北京100071(张兆山) |
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| 摘 要： | 我们采用基因工程抗体制备技术，从分泌抗人 ｂ Ｆ Ｇ Ｆ单抗的鼠杂交瘤细胞成功地克隆了其轻链可变区基因，并对其进行了序列测定及抗体基因结构分析。这为构建 ｂ Ｆ Ｇ Ｆ单链抗体，防止 ｂ Ｆ Ｇ Ｆ引起的副作用及进行体内定位诊断等研究打下了基础。
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| 关 键 词： | 碱性成纤维细胞生长因子 轻链可变区基因 克隆及测序 |
Cloning and Sequencing of the Light Chain Variable Region Gene of Anti bFGF Mouse Monoclonal Antibody |
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| LIU Chunjie ,WANG Dewen ,ZHANG Zhaoshan ,GAO Yabing ,XIONG Chengqi ,ZHENG Zhaorong.Cloning and Sequencing of the Light Chain Variable Region Gene of Anti bFGF Mouse Monoclonal Antibody[J].Chinese Journal of Preventive Veterinary Medicine,1999,21(5):359-362. |
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| Authors: | LIU Chunjie WANG Dewen ZHANG Zhaoshan GAO Yabing XIONG Chengqi ZHENG Zhaorong |
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| Institution: | LIU Chunjie 2,WANG Dewen 1,ZHANG Zhaoshan 3,GAO Yabing 1,XIONG Chengqi 1,ZHENG Zhaorong 2 |
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| Abstract: | We have cloned and sequenced the light chain variable region gene of mouse anti human basic fibroblast growth factor(bFGF)monoclonal antibody from morse hybridoma cell with genetic engineering antibody preparation technique,and analysed the structure of the light chain variable region gene.These provide a basis for further construction of anti bFGF single chain Fv fragment,which is useful for studing on location diagnose in vivo and preventing of bFGF side effect. |
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| Keywords: | Basic fibroblast growth factor(bFGF) Light chain variable region gene Clone and sequence |
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