| 金合欢组织培养技术研究 |
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| 引用本文： | 贾文庆,刘会超,郝俊富.金合欢组织培养技术研究[J].广东农业科学,2012,39(2):40-41. |
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| 作者姓名： | 贾文庆 刘会超 郝俊富 |
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| 作者单位： | 河南科技学院园林学院 |
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| 基金项目： | 国家科技部科技人员服务企业行动项目(2009GJD00045) |
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| 摘 要： | 以金合欢的种子为外植体进行离体培养,初步建立了金合欢离体培养再生体系。结果表明:最适宜诱导愈伤组织和不定芽的培养基是MS+6-BA 1.5 mg/L+NAA 0.1 mg/L;最适宜的增殖培养基是MS+6-BA 2.0 mg/L+NAA 0.2 mg/L;最适宜诱导生根的培养基是1/2MS+IBA 0.4 mg/L。
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| 关 键 词： | 金合欢 无菌苗 不定芽 增殖培养 |
Study on tissue culture of Acacia dealbat |
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| JIA Wen-qing,LIU Hui-chao,HAO Jun-fu.Study on tissue culture of Acacia dealbat[J].Guangdong Agricultural Sciences,2012,39(2):40-41. |
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| Authors: | JIA Wen-qing LIU Hui-chao HAO Jun-fu |
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| Institution: | (Landscape College,Henan Institute of Science and Technology,Xinxiang 453003,China) |
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| Abstract: | The seeds were used as explant to carry out tissue culture of Acacia dealbat,the system of efficient plant regenerations was established initially.The results showed that the explants of Albizzia treated by 0.15% HgCl2 for 10 min could obtained low rate of dead and infection,straight insertion seed is best inoculation method.The optimal culture mediums for callus tissue and adventitious bud induction was MS+6-BA 1.5 mg/L+NAA 0.1 mg/L,the optimal enrichment medium was MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,the optimal rooting medium was 1/2MS+IBA 0.4 mg/L. |
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| Keywords: | Albizzia aseptic seedling adventitious bud multiplication culture |
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