决明子叶愈伤组织中大黄素与大黄酚含量的测定

决明子为豆科植物决明（Cassia obtusifolia L．）或小决明（Cassia tora L．）的干燥成熟种子，具有降血压、降血脂、保肝和抑菌等功效，其主要有效成分为蒽醌类化合物（大黄素、大黄酚等）。笔者利用愈伤组织诱导培养的方法，拟提高决明材料中蒽醌类化合物的含量，为研究蒽醌类有效成分的生物合成途径以及用简单培养方法获得有效成分提供依据。

Determination of Emodin and Chrysophanol Contents. in Callus of Cassia tora L. Leaf

Objective] Through inductive culture, emedin and ehrysophanol contents in cassia seed were increased. Method]MS culture medium was used to bourgcon seedlings of cassia seed and to induce cotyledon callus of cassia seed while HPLC method was adopted to determine emodin and chry-sophanol contents. Result] Emedin and chrysophanol contents in cotyledon callus were 0.099% and 0.312%, respectively, while they were 0.029% and 0.190% respectively in cassia seed. Conclusion]The method of inducing cotyledon callus was helpful for increasing emedin content in cassia seed.