利用BSA-Seq方法鉴定向日葵耐盐候选基因

挖掘向日葵耐盐基因对作物耐盐品种选育具有重要的意义。本研究利用高耐盐自交系Y07-136R（父本）和不耐盐自交系Y05-222A（母本）及其构建的包含600个单株的F2分离群体为试验材料，挑选分离群体中耐盐和不耐盐的极端分离株系各50株单株，分别构建两个DNA池用于高通量测序以分析筛选向日葵耐盐候选基因。通过本研究共发掘到6个耐盐关键候选基因（Ha0_73Ns.730/Ha10.228/Ha12.2377/Ha2.3822/Ha8.182/Ha9.5434），功能注释分别为依赖ATP的RNA解旋酶、热休克蛋白、生长素结合蛋白、溶质运载蛋白家族成员、核糖体蛋白S21家族成员及未知功能蛋白。通过候选基因同源分析、代谢通路分析及共表达基因分析发现，这些基因参与植物逆境胁迫调控，可能具有耐盐的生物学功能。本研究结果为耐盐基因的克隆及功能解析提供了重要候选基因，同时为向日葵耐盐新品种的选育提供了重要的分子标记。

Identification of major salt-tolerant genes via BSA-Seq method in sunflower

Sunflower is known as the pioneer crop in saline soil. Identification of salt-tolerant genes from sunflower has great significance to crop salt-tolerance breeding. In this study, an F2 population with 600 individuals was constructed using salt-tolerant line Y07-136R and salt-sensitive line Y05-222A; 50 F2 salt-tolerant individuals and 50 salt-sensitive individuals were selected respectively to construct 2 DNA pools which were used for high-throughput sequencing. The key candidate genes associated with salt-tolerant genes were identified via BSA-Seq. The results showed that, total 6 key salt-tolerant genes, Ha0_73Ns.730, Ha10.228, Ha12.2377, Ha2.3822, Ha8.182 and Ha9.5434, were identified and the annotation functions were ATP-dependent RNA helicase, heat shock protein, auxin-binding protein, solute carrier family 35 members, putative ribosomal protein S21 family protein and uncharacterized protein. The results of candidate genes homology analysis, metabolic pathway analysis and co-expression analysis showed that these 6 candidate genes may be involved in plant stress regulation. This research provided key salt-tolerant candidate genes and also provides important markers for salt-tolerant breeding in sunflower.