| 1株竹鼠奇异变形杆菌的分离鉴定及药敏分析 |
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| 引用本文： | 翟颀,翟少伦,吕殿红,温肖会,吴国景,霍玮,涂杜,魏文康,贾春玲,周秀蓉.1株竹鼠奇异变形杆菌的分离鉴定及药敏分析[J].中国动物传染病学报,2021(1). |
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| 作者姓名： | 翟颀 翟少伦 吕殿红 温肖会 吴国景 霍玮 涂杜 魏文康 贾春玲 周秀蓉 |
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| 作者单位： | 广东省农业科学院动物卫生研究所 |
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| 基金项目： | 广东省重点研发项目(2019B020217002);广东省现代农业产业技术体系创新团队建设专项资金(2019KJ119);广东省农业科学院学科团队建设(201634TD);广东省农业科学院院长基金(202024);广东省2018年乡村振兴战略专项资金(粤农计2018-54);清远市科技计划项目(DZXQY017) |
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| 摘 要： | 为确定广东某竹鼠养殖场发生竹鼠死亡的原因,本研究从发病竹鼠血液和组织中分离到可疑细菌,并对分离的菌株进行革兰氏染色、生化特性分析和16S rRNA基因鉴定及序列分析,同时进行药敏试验。结果显示:分离的细菌为杆状、球状和球杆状形状不一的革兰氏阴性菌;生化特性与奇异变形杆菌基本一致;16S rRNA序列与NCBI数据库中奇异变形杆菌16S rRNA核苷酸相似性为99%以上。结果表明,该分离细菌为奇异变形杆菌将其命名为GD/ZS-01株;药敏实验显示,该菌株对头孢哌酮、丁胺卡那和左氧氟沙星高度敏感。本研究结果为竹鼠养殖场疫情的防治提供了科学参考。
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| 关 键 词： | 竹鼠 奇异变形杆菌 分离鉴定 耐药性 |
Isolation,Identification and Drug Resistance of Proteus Mirabilis from Bamboo Rats |
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| ZHAI Qi,ZHAI Shaolun,LYV Dianhong,WEN Xiaohui,WU Guojing,HUO Wei,TU Du,Wei Wenkang,JIA Chunling,ZHOU Xiurong.Isolation,Identification and Drug Resistance of Proteus Mirabilis from Bamboo Rats[J].Chinese Journal of Animal Infectious Diseases,2021(1). |
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| Authors: | ZHAI Qi ZHAI Shaolun LYV Dianhong WEN Xiaohui WU Guojing HUO Wei TU Du Wei Wenkang JIA Chunling ZHOU Xiurong |
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| Institution: | (Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and RuralAffairs, Key Laboratory of Livestock Disease Prevention of GuangdongProvince, Scientific Observation and Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China) |
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| Abstract: | In order to investigate the etiological agent for deaths of bamboo rats on a farm located in Guangdong province,bacterial isolation and identification were conducted using blood and tissue samples of diseased animals.The bacterial isolate was then examined with gram staining,biochemistry test,16S rRNA sequencing and drug testing.The results showed that this isolate was gram-negative bacteria of rod or sphere shapes and its biochemistry characteristics were basically consistent with those of Proteus mirabilis.Meanwhile,this isolate was more than 99% homologous with Proteus mirabilis in NCBI database by 16S rRNA sequencing and designated as GD/ZS-01 strain.Drug testing showed that it was highly susceptible to cefoperazone,butanamine and levofloxacin.The results provided a scientific reference for the prevention and treatment of Proteus mirabilis infection in bamboo rats. |
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| Keywords: | Bamboo rats Proteus mirabilis isolation drug resistance |
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