| 马立克氏病病毒的分离和鉴定 |
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| 引用本文： | 任文陟,朱维正. 马立克氏病病毒的分离和鉴定[J]. 中国兽医学报, 1991, 0(3) |
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| 作者姓名： | 任文陟 朱维正 |
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| 作者单位： | 兽医大学传染病教研室(任文陟)，兽医大学传染病教研室(朱维正) |
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| 摘 要： | 从长春、天津、大连地区分离到10株马立克氏病病毒(MDV),经电镜观察、琼脂扩散试验、痘斑及蚀斑形成试验,均符合MDV的特点.通过MDV单克隆抗体、鸡胚细胞培养及致病性试验等鉴定,10株病毒均为血清I群MDV.火鸡疱疹病毒疫苗对其中的C-4株的保护率低于京-1株(P<0.01),而对其它株的保护率与京-1株差异不显著(P>0.05).
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| 关 键 词： | MDV 分离 鉴定 |
Isolation and Identification of Marek' s Disease Virus |
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| Ren Wenzhi et al. Isolation and Identification of Marek' s Disease Virus[J]. Chinese Journal of Veterinary Science, 1991, 0(3) |
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| Authors: | Ren Wenzhi et al |
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| Abstract: | Ten strains of Marek' s disease virus (designated as C- 1, C - 3, C- 4, C-5, C-6, C -7, C-8A, C-8D, TJ-2, DL-1) were isolated with chick embryo fibroblast cell cultures from Changchun, Tianjin and Dalian of China. The electron microscopy, agar gel precip-itin test, plaque - forming test and pock forming test showed that their biological characteristics were the same as MDV. Based upon the results of fluorescence group-specific monoclonal antibody staining, cultivation on chick embryo fibroblasts and reproduced infection in chicklings, they were identified as serogroup 1. The immunization test of HVT indicated that the protection rate for chicklings challenged with C- 4 strain was much lower than that of those with Jing-1 strain (P<0.01), but there were no statistical differences between the protection rates for Jing - 1 strain and for the others. |
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| Keywords: | Marek's desease virus (MDV) isolation identification |
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