| 荔枝多糖的分离纯化及清除自由基作用研究 |
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| 引用本文： | 李巍巍,张名位,魏振承,张瑞芬,唐小俊,张雁,邓元媛.荔枝多糖的分离纯化及清除自由基作用研究[J].广东农业科学,2009(5):124-127. |
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| 作者姓名： | 李巍巍 张名位 魏振承 张瑞芬 唐小俊 张雁 邓元媛 |
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| 作者单位： | 李巍巍(华中农业大学食品科技学院,湖北武汉,430070;广东省农科院生物技术研究所/农业部功能食品重点开放实验室,广东广州,510610);张名位,魏振承,张瑞芬,唐小俊,张雁,邓元媛(广东省农科院生物技术研究所/农业部功能食品重点开放实验室,广东广州,510610) |
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| 基金项目： | 广东省自然科学基金团队项目 |
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| 摘 要： | 研究了荔枝多糖(Litchi Polysaccharides,简称LCP)及其纯化后的多糖组分清除自由基作用,LCP经DEAE-Cellulose 52柱色谱分级获得4个多糖组分LCP-1、LCP-2、LCP-3和LCP-4,以Vc为对照,测定了LCP及其4个多糖组分对.OH、O2-.和DPPH的清除能力.结果表明,LCP及4个多糖组分均有清除.OH、O2-和DPPH的能力,其中LCP-2的清除作用最强,其清除.OH、O2-.和DPPH的能力分别相当于Vc的22.6%、44.2%、10.7%.
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| 关 键 词： | 荔枝多糖 分离纯化 清除自由基 |
Study on purification and scavenging free radical effects of Polysaccharides from Litchi |
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| LI Wei-wei,ZHANG Ming-wei,WEI Zhen-cheng,ZHANG Rui-fen,TANG Xiao-jun,ZHANG Yan,DENG Yuan-yuan.Study on purification and scavenging free radical effects of Polysaccharides from Litchi[J].Guangdong Agricultural Sciences,2009(5):124-127. |
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| Authors: | LI Wei-wei ZHANG Ming-wei WEI Zhen-cheng ZHANG Rui-fen TANG Xiao-jun ZHANG Yan DENG Yuan-yuan |
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| Institution: | LI Wei-wei1,2,ZHANG Ming-wei2,WEI Zhen-cheng2,ZHANG Rui-fen2,TANG Xiao-jun2,ZHANG Yan2,DENG Yuan-yuan2(1.Food Science and Technology College,Huazhong Agricultural University,Wuhan 430070,China,2.Bio-tech Research Institute,Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Food,Ministry of Agriculture,Guangzhou 510610,China) |
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| Abstract: | This paper studied the scavenging free radical effects of polysaccharides from Litchi(referred to as LCP) and purified polysaccharides.LCP were further purified into LCP-1,LCP-2,LCP-3 and LCP-4 by DEAE-Cellulose 52 column chromatography.Vc as control,the scavenging ability of.OH,O2-.and DPPH of LCP and LCP-1,LCP-2,LCP-3,LCP-4 were determinated.The results showed that LCP and LCP-1,LCP-2,LCP-3,LCP-4 had the ability of scavenging.OH,O2-.and DPPH,And the scavenging effect of LCP-2 on.OH,O2-.and DPPH was the st... |
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| Keywords: | LCP purification scavenging free radical |
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