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马铃薯抗卷叶病毒基因工程研究现状
引用本文:李云,卢其能,赵昶灵,沈春修.马铃薯抗卷叶病毒基因工程研究现状[J].黑龙江农业科学,2011(9):142-149.
作者姓名:李云  卢其能  赵昶灵  沈春修
作者单位:1. 宜春学院生命科学与资源环境学院,江西宜春,336000
2. 云南农业大学农学与生物技术学院,云南昆明,650201
基金项目:国家自然科学基金资助项目
摘    要:综述了马铃薯抗卷叶病毒基因工程的研究进展。在抗马铃薯卷叶病毒基因工程研究中,主要采用RNA干涉、转抗马铃薯卷叶病毒基因、核酶剪切基因组、基因定点突变等方法获得抗马铃薯卷叶病毒植株,已经取得突破性进展。通过对抗病基因工程策略及取得的成效进行概括总结,旨在为抗马铃薯卷叶病毒育种工作提供借鉴。

关 键 词:马铃薯卷叶病毒  基因工程  遗传转化  抗病育种

Research Advances in the Gene Engineering of Potato Resistance to Leaf roll Virus
LI Yun,LU Qi-neng,ZHAO Chang-ling,SHEN Chun-xiu.Research Advances in the Gene Engineering of Potato Resistance to Leaf roll Virus[J].Heilongjiang Agricultural Science,2011(9):142-149.
Authors:LI Yun    LU Qi-neng  ZHAO Chang-ling  SHEN Chun-xiu
Institution:LI Yun1,2,LU Qi-neng1,ZHAO Chang-ling2,SHEN Chun-xiu1(1.Life Sciences,Resources and Environment Sciences School of Yichun University,Yichun,Jiangxi 336000,2.Agronomy and Biotechnology College of Yunnan Agricultural University,Kunming,Yunnan 650201)
Abstract:The research progress on resistance genetic engineering of potato leafroll virus was summarized. In resistance genetic engineering of potato leaf roll virus,the scientists implied mainly RNA interference, transferring resistance genes,the genome was cut by ribozyme,gene site-directed mutagenesis and other methods to obtain anti-potato leafroll virus plants, a breakthrough has been achieved. This paper could provide a reference for breeding the potato with resistance to PLRV by analyzing genetic engineering strategy and the achievements.
Keywords:potato leafroll virus  gene engineering  genetic transformation  resistance breeding
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