快速溶剂萃取-高效液相色谱法测定莎稗磷残留

建立了快速溶剂萃取-高效液相色谱法测定土壤、大米中莎稗磷残留量的分析方法.样品经快速溶剂萃取,旋转蒸发,SPE-C18柱净化后,在流动相为甲醇∶水=70:30,C18柱(250 mm×4.6mm,5μm),230 nm,流速1.0mL·min-1条件下分析测定.结果表明:以甲醇为萃取剂、选择50%的冲池体积选、于100...

Residue Determination of Anilofos in Soil and Rice by Rapid Pressure Organic Solvents Extraction-High Performance Liquid Chromatography

A rapid pressure organic solvents extraction-high performance liquid chromatography method was built to determinate the residual of anilofos in soil and rice.The sample was extracted by organic solvents,rotary evaporation,by C18 solid-phase extraction.The C18 column was used and the mobile phase was a mixture of methanol∶water=70∶30.The detector was VWD and the flow rate was 1.0 mL per minute and the detection wavelength was 230 nm in the HPLC system.The results showed that samples were extracted with methanol,50% of the sample cell,100℃ and 10 min,once circle,10 mL eluent,the recoveries were 90.2%～98.3%.The relative standard deviation was less than 2.89%.The minimum detection was 0.1 mg·L-1.The standard working curve of anilofos had a good linear relation in the concentration range from 0.2～10.0 mg·L-1.This method could meet the requirements of determination because of its simple operation,small interfering and high sensitivity.