巫山淫羊藿中异戊烯基黄酮类活性成分的提取及抗氧化作用研究

目的]研究巫山淫羊藿有效成分的分离和异戊烯基黄酮类成分的抗氧化性。方法]采用柱层析分离得到各种异戊烯基黄酮类成分,以体外抗氧化体系为模型,DPPH自由基为清除对象,以VC为对照,采用分光光度法测定其抗氧化活性。结果]结果表明,巫山淫羊藿提取液及其中7种异戊烯基黄酮类单体成分对DPPH自由基均有清除作用,具有较强的抗氧化能力,其中抗氧化作用最强的为去多甲基羟基淫羊藿素,其50%的清除率与VC相当,其次为淫羊藿属苷C,并且这种对自由基清除率的高低与黄酮的结构有密切的关系。结论]该研究为巫山淫羊藿在化妆品、保健品和药品方面的使用提供科学依据。

Study on Extraction and Antioxidation Effects of Flavonoids from E.wushanense

Objective]The purpose was to study the extraction of effective components from E.wushanense and oxidation resistance of flavonoids(diphylloside A,epimedoside A,epimedinC,icariin,epimedoside C,icarisoside A and desmethylanhydroicaritin).Method]The column chromatographic separation was used to get various flavonoids.With in vitro antioxidation system as model and DPPH free radical as scavenging object,the antioxidant activity of flavonoids was determined by spectrophotometry with VC as CK.Result] The results indicated that the extracting solution from E.wushanense and seven kinds of flavonoids had scavenging effect on DPPH free radical and strong antioxidant capacity,the antioxidant capacity of Desmethylanhydroicaritin was the strongest than other,secondly epimedoside C,which scavenging effect on DPPH free radical had close related to structure of flavonoids.Scavenging effects of the two flavonoids were high as well as VC.Conclusion]This research will provide the scientific basis for application of E.wushanense in cosmetic,health products and medicine.