3种中草药对克氏原螯虾抗菌活性的影响

将陈皮、甘草、淫羊藿3种中草药粉碎过筛后,再用醇提法分别提取其有效成分,将其制备出的浓缩液按质量分数5%的比例添加于基础饲料中制成颗粒药饵投喂克氏原螯虾,以基础饲料为对照组,研究3种中草药对克氏原螯虾抗菌活性的影响。结果表明,3种中草药能有效地抑制大肠杆菌、嗜水气单胞菌、金黄色葡萄球菌、溶壁微球菌。其中,甘草、成皮和淫羊藿对4种细菌的平均抑菌率达到59.14%、47.53%和48.65%。实验证明,甘草、陈皮和淫羊藿这3种中草药均能不同程度地激活克氏原螯虾的免疫力,可以作为免疫增强剂应用于克氏原螯虾的生产。

The Effect of Three Chinese Medicinal on Antibacterial Activity for Procambarus clarkia

Effective components were extracted from three kinds of Chinese medicinal herb,which were dried from Herba Epimedii,Glycyrrhiza uralensis and Tangerine by alcohol formulation.The basic feed as control group,5% the mass fraction of effective components were add in basic feed to feed Procambarus clarkia.To study the influence of the antimicrobial activity in P.clarkia,throwing quantity was the shrimp 2% of its body weight when feed in the late afternoon in succession for 30 days.It showed that Escherichia coli,Aeromonas hydrophilia,Staphyloccocus aureus and Micrococcus lysodeikticus were inhibited effectively and the antibacterial rate of Glycyrrhiza uralensis,Tangerine and Herba Epimedii reached 59.14%,47.53% and 48.65% respectively.The experiment proved that the three chinese herbal medicine Tangerine,Glycyrrhiza uralensis and Herba Epimedii could improve the immunity system of shrimp at different degree,and could be used as immunity intensifier for shrimp cultivation.