| 一株产漆酶细菌的分离、鉴定及对造纸黑液处理的初步研究 |
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| 引用本文： | 汪春蕾,赵敏,杨洪一,崔岱宗,李泰仑,李德斌,张宁.一株产漆酶细菌的分离、鉴定及对造纸黑液处理的初步研究[J].北京林业大学学报,2011,33(3):81-85. |
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| 作者姓名： | 汪春蕾 赵敏 杨洪一 崔岱宗 李泰仑 李德斌 张宁 |
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| 作者单位： | 东北林业大学生命科学学院 |
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| 基金项目： | 国家自然科学基金项目，黑龙江省自然科学基金项目，世界自然基金项目 |
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| 摘 要： | 利用铜离子作为筛选剂,从凉水国家级自然保护区的土样中分离纯化出一株具有高漆酶活性的细菌.通过形态学观察、生理生化特性测定及16S rDNA序列分析,鉴定其为枯草芽孢杆菌(Bacillus subtilis),命名为B.subtilis WD23.根据GenBank上公布的Baccillus subtilis cotA ...
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| 关 键 词： | 枯草芽孢杆菌 芽孢蛋白 漆酶 造纸黑液 废水处理 |
| 收稿时间： | 1900-01-01 |
Isolation and identification of a bacterium strain exhibiting laccase activity and its treatment to papermaking black liquid |
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| WANG Chun-lei,ZHAO Min,YANG Hong-yi,GUI Dai-zong,LI Tai-lun,LI De-bin,ZHANG Ning.Isolation and identification of a bacterium strain exhibiting laccase activity and its treatment to papermaking black liquid[J].Journal of Beijing Forestry University,2011,33(3):81-85. |
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| Authors: | WANG Chun-lei ZHAO Min YANG Hong-yi GUI Dai-zong LI Tai-lun LI De-bin ZHANG Ning |
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| Institution: | College of Life Sciences, Northeast Forestry University, Harbin, 150040, P. R. China. |
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| Abstract: | A novel bacterium strain exhibiting high laccase activity was separated and purified from the forest soil of the Liangshui National Nature Reserve in Heilongjiang Province with Cu2+ as the enrichment culture reagent.The strain was identified as Bacillus subtilis WD23 by morphological observation,physiological and biochemical tests and 16S rDNA sequence analysis.The cotA gene was cloned by primers designed according to B.subtilis cotA gene sequence on the GenBank.The cotA gene was inducibly expressed by the ... |
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| Keywords: | Bacillus subtilis spore coat protein laccase papermaking black liquid wastewater treatment |
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