双歧杆菌对正常小白鼠免疫调节及肠道菌群的影响

应用动物实验方法,研究婴儿双歧杆菌和长双歧杆菌对正常小白鼠免疫功能的调节作用以及对肠道菌群的影响。结果发现,两株菌对正常小白鼠体重、脾/体重和肾/体重比无显著性影响;对肝/体重比有增加趋势;能显著增加血液中IgA,IgG,C3,C4,IL-2,IL-6和IL-8的含量(P<0.05),婴儿双歧杆菌效果优于长双歧杆菌;婴儿双歧杆菌可极显著增加IgM的含量(P<0.01)。两株菌均可显著增加双歧杆菌(P<0.05)和乳杆菌的数量,显著降低肠杆菌的数量(P<0.05)。实验结果表明,两株菌对正常BALB/C小白鼠的免疫功能具有一定的正相调节作用,并具有良好调节肠道菌群能力,婴儿双歧杆菌效果优于长双歧杆菌。

The effect of Bifidobacterium on immunity and intestinal microflora of normal mouse

The immunomodulation of B. infantis and B. longum on the immunity of normal mouse, together with the effect of B. infantis and B. longum on the intestinal microflora were investigated. It was found that two strains had no significant effects on weight, spleen/weight and kidney/weight. There was increase trend in respect of live/weight. IgA, IgG, C3, C4, IL-2, IL-6 and IL-8 in serum increased significantly(P <0.05). Meanwhile the effects of B. infantis were better than that of B. longum. IgM increased significantly(P <0.01)when mouse were treated with B. infantis. The amounts Bifidobacterium and Lactobacillus in colon and appendix contents were improved significantly(P <0.05), and the amounts of Enterobacteriaceae went down significantly(P <0.05) when mouse were treated with Bifidobacterium. The results showed that immune functions of normal mouse treated with B. infantis and B. longum were positively modulated to certain extent and the two strains had good ability of modulation on intestinal microflora. The effects of B. infantis were better than that of B.longum.