| 短雄花序板栗芽变的AFLP分析 |
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| 引用本文： | 徐月,曹庆芹,冯永庆,杨凯,秦岭,廖康.短雄花序板栗芽变的AFLP分析[J].园艺学报,2006,33(6):1321-1324. |
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| 作者姓名： | 徐月 曹庆芹 冯永庆 杨凯 秦岭 廖康 |
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| 作者单位： | ( 北京农学院植物科学技术系, 北京102206; 北京农学院生物技术系, 北京102206; 新疆农业大学园艺学院, 新疆乌鲁木齐830052) |
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| 基金项目： | 北京市自然科学基金资助项目(6052004)，北京市属市管高校人才强教计划资助项目 |
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| 摘 要： | 利用AFLP分子标记技术对板栗短雄花序芽变及其母株进行了初步研究, 72种引物组合共产生5 129条清晰谱带, 多态性位点53个, 多态性2.05% , 相似性系数0.9897, 差异片段主要集中于300～900 bp之间。研究表明短雄花序芽变和对照个体之间变异与遗传物质的改变相关, 其中一些可能与雄花序变短的基因有关。
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| 关 键 词： | 板栗 芽变 短雄花序 AFLP |
| 文章编号： | 0513-353X（2006）06-1321-04 |
| 收稿时间： | 2006-02-21 |
| 修稿时间： | 2006-02-212006-07-28 |
AFLP Analysis on the Short Catkins Mutation of Chestnut |
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| Xu Yue,Cao Qingqin,Feng Yongqing,Yang Kai,Qin Ling,Liao Kang.AFLP Analysis on the Short Catkins Mutation of Chestnut[J].Acta Horticulturae Sinica,2006,33(6):1321-1324. |
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| Authors: | Xu Yue Cao Qingqin Feng Yongqing Yang Kai Qin Ling Liao Kang |
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| Institution: | (Deparment of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China; Deparment of Biology Technology, Beijing University of Agriculture, Beijing 102206, China; Institute of Horticulture, Xinjiang Agricultural University, Urumchi, Xinjiang 830052, China) |
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| Abstract: | AFLP was used to compare the short catkins sport and its mother plant in chestnut(Castanea mollissima Bl.).A total of 72 selective primer pairs amplified 5 129 clear bands,of those 53 were polymorphic loci.The polymorphic rate and Nei's association coefficient between them were 2.05% and 0.9897 respectively.The molecular weight range of the polymorphic fragments was mainly 300-900 bp.The results indicated that there were some molecular-level differentiation between chestnut and its short catkins sport.Some of the specific segments had been considered as markers of short catkins in chestnut. |
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| Keywords: | Chestnut Sport Short catkin AFLP |
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