西瓜幼苗多倍体诱导方法研究

目的]获得西瓜多倍体,并提高西瓜多倍体的诱变率。方法]对3个二倍体西瓜种质进行秋水仙素滴苗、不同苗态摘心、低温等综合处理诱变多倍体。结果]结果表明:通过对各个单因素进行试验,初步确定了正交试验的3个水平。秋水仙素浓度为0.2%~0.4%,处理低温分别为12、15、18℃,苗态处理为1叶,2叶,3叶1心摘心处理,处理时间为4~6 d时,提高西瓜多倍体的诱变率效果较好。结论]对西瓜幼苗进行摘心处理诱变能显著提高多倍体的诱变率。

Study on Polyploid Induction Methods of Watermelon Seedling

Objective] The research aimed to obtain watermelon polyploidy and increase its induction rate.Method] Comprehensive treatments such as dripping plantlets with colchicine,top removal in different seedling states and low-temperature were conducted on 3 diploid watermelon germplasm to induce polyploidy.Result] The results indicated that 3 levels in orthogonal experiment were preliminarily confirmed through experiment on each single factor.When colchicine concentration was at 0.2%～0.4%,low temperatures in treatment were 12,15 and 18 ℃,seedling states of removaling top were at first leaves,two leaves and trefoil and one top-leaf and time was 4～6 d,its effect of increasing induction rate in watermelon polyploidy was better.Conclusion] Induction with top removal treatment on watermelon seedling could increase the induction rate of polyploidy.