| 从福尔马林保存的牙鲆中克隆的18S rDNA进行系统发育分析的可靠性 |
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| 引用本文： | 鲍宝龙 杨桂梅 龚小玲 黄连安 任大明. 从福尔马林保存的牙鲆中克隆的18S rDNA进行系统发育分析的可靠性[J]. 上海水产大学学报, 2004, 13(3): 193-197 |
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| 作者姓名： | 鲍宝龙 杨桂梅 龚小玲 黄连安 任大明 |
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| 作者单位： | [1]上海水产大学生命科学与技术学院，上海200090 [2]复旦大学生命科学学院，上海200433 |
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| 基金项目： | 上海市高等学校发展基金项目(科2000-104)，上海市科委重点学科项目资助 |
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| 摘 要： | 从福尔马林保存的牙鲆和活体牙鲆肌肉组织中，利用基因拼接的方法首次克隆到1205bp和1295bp的长片段序列。福尔马林保存标本与活体标本DNA序列的同源率为82％；和活体标本的18S rDNA序列相比，标本DNA的碱基缺失率为7％，碱基替换比例为11％。利用福尔马林标本的18S rDNA同源性高的区域进行系统发育分析，表明其结果是可信的。
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| 关 键 词： | 牙鲆 18S rDNA 福尔马林保存 系统发育分析 |
| 文章编号： | 1004-7271(2004)03-0193-05 |
| 修稿时间： | 2004-02-24 |
On the rationality of phylogenetic analyses of 18S rDNA cloned from formalin-fixed Paralichthys olivaceus |
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| BAO Bao-long,YANG Gui-mei,GONG Xiao-ling,HUANG Lian-an,REN Da-ming. On the rationality of phylogenetic analyses of 18S rDNA cloned from formalin-fixed Paralichthys olivaceus[J]. Journal of Shanghai Fisheries University, 2004, 13(3): 193-197 |
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| Authors: | BAO Bao-long YANG Gui-mei GONG Xiao-ling HUANG Lian-an REN Da-ming |
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| Affiliation: | BAO Bao-long~1,YANG Gui-mei~1,GONG Xiao-ling~1,HUANG Lian-an~1,REN Da-ming~2 |
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| Abstract: | Because some species of fishes have been becoming more and more difficult to be collected, many phylogenetic researchers have no other choice but adopt formalin-fixed specimens. There are many reports on molecular phylogenetic analyses based on DNA sequences from formalin-fixed specimens, but seldom researches have discussed the reliability about the DNA sequences from formalin-fixed specimens in applying phylogenetic analysis. Here the partial sequences, 1205bp and 1295bp 18S rDNA we reported, were first cloned form muscle of formalin-fixed and live Paralichthys olivaceus by using gene overlap strategy. Compared with live specimen, 80% bases of 18S rDNA sequence are identical, 7% are deleted, and 11% are replaced in formalin-fixed specimen. The DNA sequences from formalin-fixed and live specimens were used simultaneously in phylogenetic analysis based on high homology area. We think it is reliable in high-class molecular phylogenetic analysis. |
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| Keywords: | Paralichthys olivaceus 18S rDNA formalin-fixed phylogenetic analyses |
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