| 黑木耳菌糠粗纤维素酶和木聚糖酶浸提条件研究 |
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| 引用本文： | 马怀良,龚振杰,陈欢,弥春霞,杨楠.黑木耳菌糠粗纤维素酶和木聚糖酶浸提条件研究[J].北方园艺,2010(20). |
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| 作者姓名： | 马怀良 龚振杰 陈欢 弥春霞 杨楠 |
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| 作者单位： | 牡丹江师范学院生命科学与技术学院; |
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| 基金项目： | 国家科技部科技人员服务企业行动资助项目 |
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| 摘 要： | 在其它浸提条件一致的情况下,分别选取不同时间、不同温度、不同料液比进行粗酶液制备,测定羧甲基纤维素酶活力;在单因素试验基础上,以羧甲基纤维素酶活力和木聚糖酶活力为指标,进行三因素三水平L9(33)正交试验,研究浸提黑木耳菌糠羧甲基纤维素酶、木聚糖酶的最佳条件。结果表明:黑木耳菌糠在高速组织捣碎机内10 000 r/min匀浆2 min,可使菌糠中的菌丝断裂,有利于酶的释放;羧甲基纤维素酶浸提最佳条件为温度30℃、时间1.0 h、液料比40∶1;木聚糖酶浸提最佳条件为温度25℃、时间2.0 h、液料比50∶1。
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| 关 键 词： | 纤维素酶 木聚糖酶 黑木耳 菌糠 浸提 |
Study on Conditions of Extracting Crude CMCase and Xylanase from Auricularia auricula-judae Residue |
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| MA Huai-liang,GONG Zhen-jie,CHEN Huan,MI Chun-xia,YANG Nan.Study on Conditions of Extracting Crude CMCase and Xylanase from Auricularia auricula-judae Residue[J].Northern Horticulture,2010(20). |
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| Authors: | MA Huai-liang GONG Zhen-jie CHEN Huan MI Chun-xia YANG Nan |
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| Institution: | MA Huai-liang,GONG Zhen-jie,CHEN Huan,MI Chun-xia,YANG Nan(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang,Heilongjiang 157012) |
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| Abstract: | The other extraction conditions in the same situation,were selected at different times,different temperatures and different liquid ratio for crude enzyme preparation,the carboxymethyl cellulase activity was mensurated,based on single factor experiments to carboxymethyl cellulase and xylanase activity as an index,the best conditions of the extracting Auricularia auricula-judae residues carboxymethyl cellulase,xylanase.The results showed that the Auricularia auricula-judae residues were homogenized 2 minutes ... |
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| Keywords: | CMCase xylanase Auricularia auricula-judae residue extraction |
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