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| 高致病性猪繁殖与呼吸综合征病毒(H-PRRSV)Nsp2基因的变异监测 | |
| 引用本文： | 欧阳康,马玲,吴健敏,白安斌,邓世杰,谢彬,袁书智. 高致病性猪繁殖与呼吸综合征病毒(H-PRRSV)Nsp2基因的变异监测[J]. 西南农业学报, 2011, 24(4) |
| 作者姓名： | 欧阳康 马玲 吴健敏 白安斌 邓世杰 谢彬 袁书智 |
| 作者单位： | 1. 广西壮族自治区兽医研究所,广西南宁,530001 2. 惠朋动物药业有限责任公司,广西南宁,530001 3. 北京博莱德利生物技术有限公司,北京,100850 |
| 基金项目： | 广西自然科学基金项目(桂科攻0815009-3,桂科自0991219,桂科青0991064); 农业科技跨越计划专项资金(2009跨21) |
| 摘 要： | 收集2006 ～2010年来自广西12个地市的21份高致病性猪繁殖与呼吸综合征(H-PRRSV)阳性病料,克隆包含缺失区的Nsp2基因片段并登陆Genbank(登录号为HQ015350-HQ015370),经序列比较发现,该21株Nsp2序列具有与我国2006年爆发的H -PRRSV完全一致的缺失特征,广西H-PRRSV流行毒株Nsp2基因随着流行时间的推移朝着偏离猪高致病性蓝耳病代表毒株JXA1株的方向发展,在遗传进化树上呈地城性分布.此外,广西H-PRRSV毒株氨基酸逐渐发生变异,特别是2010年来源于3个不同地方的3个毒株5个位点均发生突变,即P19L、R33Q、R34F、,S431、E107K,这些位点的突变导致二级结构、亲水性、抗原指数和暴霉区域发生了变化. |
| 关 键 词： | 猪繁殖与呼吸综合征病毒(H-PRRSV) Nsp2基因 变异 |
Monitoring Variation of Nsp2 Genes of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus |
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| OU YANG Kang,MA Ling,WU Jian-min,BAI An-bin,DENG Shi-jie,XIE Bin,YUAN Shu-zhi. Monitoring Variation of Nsp2 Genes of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus[J]. Southwest China Journal of Agricultural Sciences, 2011, 24(4) | |
| Authors: | OU YANG Kang MA Ling WU Jian-min BAI An-bin DENG Shi-jie XIE Bin YUAN Shu-zhi |
| Affiliation: | OU YANG Kang1,MA Ling1,WU Jian-min1,BAI An-bin1,DENG Shi-jie1,XIE Bin2,YUAN Shu-zhi3(1.Guangxi Veterinary Institute,Guangxi Nanning 530001,China,2.Huipeng Animal Pharmaceutical Co.Ltd,3.Blood Biotech Co.Ltd,Beijing 100850,China) |
| Abstract: | This study collected 21 positive samples of highly pathogenic porcine reproductive and respiratory syndrome during 2006 to 2010 from 12 cities of Guangxi,the Nsp2 genes fragment including missed areas were cloned,sequenced and uploaded to Genbank,accession numbers were HQ015350-HQ015370.Through analyzing the sequences,we found that the obtained 21 Nsp2 sequences from this study both had emergence of two parts missing,it was the same as H-PRRSV strains outbreak in China in 2006,also found that Nsp2 gene of H... |
| Keywords: | Porcine reproductive and respiratory syndrome virus Nsp2 gene Variation |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |