| 利用NaN3诱变“中育5号”选育变异种质系及SSR分析 |
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| 引用本文： | 张希太,谢淑芹,张彦波,肖磊,张清华.利用NaN3诱变“中育5号”选育变异种质系及SSR分析[J].西南农业学报,2011,24(4). |
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| 作者姓名： | 张希太 谢淑芹 张彦波 肖磊 张清华 |
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| 作者单位： | 邯郸市农业科学院生物技术中心,河北邯郸,056001 |
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| 基金项目： | 河北省邯郸市科技发展计划项目“利用基因突变技术选育小麦新品种与新资源”(041068) |
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| 摘 要： | 为创造小麦新种质系,利用诱变剂叠氮化钠并采用“种子处理同时鼓入空气”、“颖苞内滴加”、“生长点注射”3种方法诱变小麦“中育5号”.其中“种子处理同时鼓入空气”方法处理的“中育5号”种子出苗率高,M1代性状变异类型丰富,变异率高,是适合小麦“中育5号”诱变处理的好方法.通过对诱变后代的系统选择,选出了YZY5-1、YZY5-2、YZY5-3 3个稳定的新种质系；利用SSR分子标记检测了“中育5号”及其变异种质系的基因组DNA,引物xgwm148、xgwm428、xgwm260、xgwm469检测出了丰富的多态性片段,从分子水平上证明了叠氮化钠对“中育5号”的诱变效果.
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| 关 键 词： | 叠氮化钠 诱变 中育5号 后代变异 SSR分新 |
New Wheat Germplasms from Variational Descendants of' Zhongyu5'Induced by NaN3 and Assessment of Genetic Diversity Using SSR Markers |
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| ZHANG Xi-tai,XIE Shu-qin,ZHANG Yan-bo,XIAO Lei,ZHANG Qing-hua.New Wheat Germplasms from Variational Descendants of' Zhongyu5'Induced by NaN3 and Assessment of Genetic Diversity Using SSR Markers[J].Southwest China Journal of Agricultural Sciences,2011,24(4). |
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| Authors: | ZHANG Xi-tai XIE Shu-qin ZHANG Yan-bo XIAO Lei ZHANG Qing-hua |
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| Institution: | ZHANG Xi-tai,XIE Shu-qin,ZHANG Yan-bo,XIAO Lei,ZHANG Qing-hua(Biotechnology Center,Handan Academy of Agricultural Sciences,Hebei Handan 056001,China) |
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| Abstract: | In order to create the new wheat germplasms,'Zhongyu5'was induced by NaN3 with three ways,including in'seed treated by NaN3 with adding air','NaN3 put in glume','NaN3 injected into the growing point in stem tip'.The results showed that 'seed treated by NaN3 with adding air'was the best way to induce'Zhongyu5'.By this way,'Zhongyu5'had high rate of emergence and the variation rate of M1 plants were the highest and the variation type were plenty.Three unaltered germplasm lines including in YZY5-1,YZY5-2 and Y... |
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| Keywords: | NaN3 Induced variation Zhongyu5 Variation descendants SSR markers |
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