牛-兔种间重组胚体外发育能力的研究

 以超排的青年母兔和经产母兔卵母细胞为受体细胞,以处于休眠期和非休眠期的黑毛和牛耳成纤维细胞为核供体,共获得180枚种间重组胚,采用M199+10%FBS和RD+10%FBS2种培养液进行体外培养,有112枚发育到2-细胞期(62.2%),26枚发育到桑椹胚期(14.4%),20枚发育到囊胚期(11.1%)。结果显示,供体细胞处于休眠期与否,对重组胚的卵裂率无显著影响(P<0.05),但以休眠期细胞为核供体的重组胚8-细胞～16-细胞胚的发育率及桑椹胚发育率显著高于非休眠期细胞的重组胚(P<0.05),二者在

Study on Development of Cloned Embryo Using Bovine Somatic Cell and Rabbit Oocyte in vitro

Total 180 reconstituted embryos were produced by using Japan Black Cattle's ear fibroblast cell whichstaying at G0 or non-G0 cell cycle stage as donor and oocyte superovulated from multiparous or young rabbit as recipient.After cultured in two kinds of medium: M199 10?S or RD 10?S, 112 of them developed to 2-cell stage (62.2%), 26developed to morula stage (14.4%), 20 developed to blastocyst stage (11.1%). The result indicated that no significantdifference was obsearved in cleavage rate between two groups of reconstituted embryo which using G0-stage cell and non-G0 stage cell as donor respectively, but when used G0-stage cell as donor, 8-cell-16-cell rate and morula rate were raisedsignificantly (P<0.05), blastocyst rate was raised significantly (P<0.01). No significant difference in cleavage rate, morularate and blastocyst rate was obsearved when using two kinds of culture medium (P>0.05).