Somatic embryogenesis in cotyledons of Picea abies is enhanced by an adventitious bud-inducing treatment |
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Authors: | Marie-Anne P Lelu Michel P Boulay Chris H Borivman |
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Institution: | (1) Research Division, Hilleshög AB, Box 302, S-26123 Landskrona, Sweden;(2) Present address: INRA, Centre de Recherches d'Orleans, Ardon, F-45160 Olivet, France;(3) LVMH Recherche Groupe Louis Vuitton-Moet-Hennessy, 50 rue de Seine, F-92704 Colombes, France |
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Abstract: | Somatic embryos were obtained from young, isolated cotyledons of Picea abies. A bud-inductive treatment for one week before transfer to a callus induction medium, resulted in a tenfold increase in the percentage of cotyledons in which embryonic tissues could be induced. The degree of expression of somatic embryogeny depends on whether cotyledons are first subjected to a callus-inducing medium or first exposed to a cytokinin treatment followed by a callus-inducing medium. It is hypothesized that two populations of cells exist, both with the inherent potential of becoming embryogenic after treatment with plant growth regulators. |
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Keywords: | Norway spruce tissue culture |
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