| TcLr24小麦STK类抗病基因同源片段的分离和特征分析 |
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| 引用本文： | 张娜,杨文香,刘大群.TcLr24小麦STK类抗病基因同源片段的分离和特征分析[J].华北农学报,2010,25(5). |
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| 作者姓名： | 张娜 杨文香 刘大群 |
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| 作者单位： | 河北农业大学植物保护学院植物病理系;河北省农作物病虫害生物防治工程技术研究中心; |
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| 基金项目： | 国家重点基础研究发展计划("973"计划)项目，国家自然科学基金资助项目，河北省自然科学基金 |
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| 摘 要： | 利用根据STK类植物抗病基因类似序列保守结构设计的引物,对TcLr24小麦叶片cDNA进行扩增,通过RT-PCR扩增得到STK类抗病基因同源片段长度811bp,开放阅读框长804bp,编码268个通读的氨基酸序列,大小29.6kDa,理论等电点6.14,该氨基酸序列与多个小麦和燕麦蛋白激酶序列高度同源,含有激酶特征结构疏水信号序列及胞外结构域。
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| 关 键 词： | 小麦叶锈病 TcLr24 抗病基因同源序列 丝氨酸/苏氨酸蛋白激酶 |
Isolation and Characterization of STK Homologue from Wheat TcLr24 |
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| ZHANG Na,YANG Wen-xiang,LIU Da-qun.Isolation and Characterization of STK Homologue from Wheat TcLr24[J].Acta Agriculturae Boreali-Sinica,2010,25(5). |
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| Authors: | ZHANG Na YANG Wen-xiang LIU Da-qun |
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| Institution: | ZHANG Na,YANG Wen-xiang,LIU Da-qun ( College of Plant Protection,Biological Control Center of Plant Diseases and Pests of Hebei Province,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | The primers were designed according to the conserved sequence of resistance gene analogs and were used to clone cDNA of TcLr24. A 811 bp STK homologous fragment was isolated from wheat leaf of TcLr24 by RT-PCR amplification. The sequencing results showed that there were 804 bp open reading frame. The deduced amino acid sequence was 268 in length,and molecular weight and iso-electric point of the protein were 29. 6 kDa and 6. 14,respectively. The STK fragment was highly homologous with several kinases from w... |
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| Keywords: | Wheat leaf rust TcLr24 Resistance gene analogs STK |
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