A useful method for preparing microsclerotial inoculum of Verticillium dahliae |
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Authors: | Toshimasa Shiraishi Hiroshi Sakai Kentaro Ikeda Toshihiko Urushibara |
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Affiliation: | 1. Gunma Agricultural Technology Center, Gunma, 493 Nishiobokata, Isesaki, Gunma, 379-2224, Japan 2. Gunma Prefectural Office, 1-1-1 Ote, Maebashi, Gunma, 371-8570, Japan
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Abstract: | Various methods of inoculating plants with Verticillium dahliae have been described; however, so far, there are no effective preparations of its microsclerotia, a major source of infection under natural field conditions. In this study, we developed a simple and effective method to prepare microsclerotial inoculum of V. dahliae using a cellophane medium that effectively promoted microsclerotia formation. In addition, a sieving process allowed the preparation of inoculum exclusively composed of microsclerotia. Our novel method provides a reproducible and stable inoculum for disease induction by V. dahliae under conditions that approximate natural infections. |
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