Chemical constituents from the fibrous root of Ophiopogon japonicus,and their effect on tube formation in human myocardial microvascular endothelial cells |
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| Institution: | 1. Shanghai University of Traditional Chinese Medicine Engineering Research Center of Modern Preparation Technology of TCM, Ministry of Education, Room 1107, No. 1200 Cai Lun Road, Zhangjiang High-Tech Park, Pudong, Shanghai, China;2. College of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, No. 1200 Cai Lun Road, Zhangjiang High-Tech Park, Pudong, Shanghai, China;3. Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Department of Pharmacy, Ruijin Road (No. 2) No. 197, Shanghai, China;1. School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei, 050017, PR China;2. Department of Pharmacy, Hebei General Hospital, Shijiazhuang, Hebei, 050051, PR China;1. Department of Pharmacology, Harbin Medical University-Daqing, Daqing, Heilongjiang 163319, China;2. Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110847, China;3. Department of Nursing, Harbin Medical University-Daqing, Daqing, Heilongjiang 163319, China |
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| Abstract: | In an effort to identify novel active constituents on the cardiovascular system, a systematic study on macroporous resin adsorption of extracts from the fibrous root of Ophiopogon japonicus was performed in a human myocardial microvascular endothelial cell line (HMMEC) based assay. One novel spirostan, named ophiogenin (1), together with six known spirostans (4–8, 10), and one new sesquiterpene glycoside, named ophioside A (2), together with one known monoterpene glycoside (9) were isolated from the active fractions, and the aglycone of compound 2 that was a new natural compound was obtained from the acid hydrolysis of 2, named ophiopogonol (3). Their structures were determined by spectral and chemical analyses. Furthermore, their effect on HMMEC tube formation was also determined. Our results indicated that compounds 4 and 8 could significantly improve the tube formation and 2 showed moderate increasing effect, while compound 5 exhibited potent inhibitory effect. |
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