分子标记在林业辅助选择育种中的应用

本文主要阐述分子标记在林木辅助选择育种中的应用。利用多种分子标记(RAPD,RFLP,AFLP,STS,SSR,STR等),可以在林木早期生长阶段对一些性状进行鉴别, 构建单种分子标记遗传连锁图谱或几种分子标记共存的混合连锁图谱和对控制数量性状的基因进行定位, 对林木群体遗传结构、遗传变异、遗传分化和基因流动进行研究, 在基因工程中, 能够追踪目的基因行为和对控制质量性状的基因进行鉴别, 对单株进行指纹图谱, 对种子质量进行监测和对品系、品种和无性系进行鉴定。

Application of Molecular Markers in Marker assisted Selection of Tree Breeding

This review paper gives an introduction to the application of molecular markers in marker assisted selection of tree breeding in the United States. At the early stage of tree growth, it becomes possible to identify certain characters, structure genetic linkage map marked by single or multiple moleculars, and position gene loci which control quantitative traits using multiple molecular markers, such as RAPD, RFLP, AFLP, STS, SSR, STR and etc., in order to study genetic structure of populations, genetic variation, genetic differentiation and gene flow of forest trees. In gene engineering molecular markers can be used to monitor behavior of target gene, identify genes which control qualitative characteristics, map finger printing and evaluate seed quality, varieties and clones of forest trees.