海洋霉菌1-B6-10代谢产物对几种镰刀菌的抑制作用

测试了海洋霉菌菌株1-B6-10代谢产物对不同镰刀菌菌丝生长、孢子萌发和显微形态的影响.结果表明,该菌代谢产物粗提物对尖孢镰刀菌Fusarium oxysporum菌株F1113、F1125和番茄镰刀菌Fusarium batatasis菌株F2016等的菌丝生长具有明显的抑制作用.当纸片上滴加0.05 mL该粗提物时,其抑菌圈直径均在32 mm以上;在菌株F1125菌丝块上直接滴加0.05 mL该粗提物,其菌丝生长抑制率达到98.39%.该代谢产物也能显著抑制尖孢镰刀菌F113和F1125孢子的萌发,用粗提物处理后培养48h,其孢子萌发率仅为8%和9.6%.显微镜观察结果表明,在添加该代谢产物亚抑菌浓度的培养基中,尖孢镰刀菌F1113菌丝的形态发生改变,部分菌丝萎缩和断裂.

Inhibition activity of the metabolite of marine mold 1-B6-10 to Fusarium spp.

The effects of the metabolite of marine mold 1-B6-10 against the mycelia growth, spore germination and morphology of Fusarium spp. were tested. The results showed that the metabolite have signifi- cantly inhibition activity to the mycelia growth of Fusarium oxysporum strain FI 113, F1125 and Fusarium batatasis strain F2016. When 0.05 mL crude extract of the metabolite was added to filter paper, the diameters of inhibition zone were exceed to 32 mm. The inhibition rate to the mycelial growth of F. oxysporum F1125 was 98.39% when 0.05 mL extract was added to the mycelium. It can also inhibit the spore germination of strain F1113 and F1125 significantly. When cultured for 48 h after treatment by the metabolite, the germination rate of spore were only 8% and 9.6% respectively. The morphological change has been observed under microscope when cultured in the medium with the sub-inhibition concentration of the metabolite. Some hyphae collapsed and broke into small segments.