| 仔猪大肠埃希氏菌、C型产气荚膜梭菌的灭活及脱毒方法研究 |
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| 引用本文： | 刘泽文,邓均华,杨克礼,梁望旺,伍锐,段正赢,周丹娜,田永祥,徐涤平.仔猪大肠埃希氏菌、C型产气荚膜梭菌的灭活及脱毒方法研究[J].安徽农业科学,2010,38(20):10702-10703,10728. |
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| 作者姓名： | 刘泽文 邓均华 杨克礼 梁望旺 伍锐 段正赢 周丹娜 田永祥 徐涤平 |
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| 作者单位： | 湖北省农业科学院畜牧兽医研究所,湖北武汉,430064 |
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| 基金项目： | 湖北省科技厅引导项目，湖北省农业科技创新中心资助项目 |
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| 摘 要： | 目的]研究仔猪大肠埃希氏菌病、C型产气荚膜梭菌病二联灭活疫苗的质量及产品的安全性。方法]对大肠埃希氏菌和C型产气荚膜梭菌浓缩菌液,用不同浓度甲醛溶液经不同灭活时间、灭活温度进行了灭活和脱毒试验。结果]大肠埃希氏菌培养物在37～38℃条件下用0.4%的甲醛溶液灭活48h,可以使细菌全部灭活;C型产气荚膜梭菌培养物在37～38℃,采用0.5%～0.8%的甲醛溶液进行灭活和脱毒7～14d,或者采用2次灭活和脱毒方法,7d可以达到完全灭活和脱毒。结论]在37～38℃条件下,用0.4%的甲醛溶液对大肠埃希氏菌培养物灭活和采用0.5%～0.8%的甲醛溶液对C型产气荚膜梭菌进行灭活和脱毒,可以达到理想效果。
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| 关 键 词： | 大肠埃希氏菌 C型产气荚膜梭菌 灭活 脱毒 |
Inactivation and Detoxification of Escherichia coli and C-type Clostridium wechii |
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| Institution: | LIU Ze-wen et al(Institute of Animal Husbandry and Veterinary,Hubei Academy of Agricultural Science,Wuhan,Hubei 430064) |
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| Abstract: | Objective] In order to assure the quality and security of Escherichia coli and C-type clostridium wechii combined vaccine.Method] The different inactive temperature and time were tested on Escherichia coli and Clostridium wechii in different concentration formaldehyde.Result] Escherichia coli was killed in 0.4% formaldehyde at 37-38 ℃ for 48 h.Clostridium wechii was inactived and losing virulence in 0.5%-0.8% formaldehyde at 37-38 ℃ for 7-14 d.Conclusion] It can get better effect to Escherichia coli in 0.4% formaldehyde at 37-38 ℃ for 48 h and 0.5%-0.8% formaldehyde at 37-38 ℃ for 7-14 d to C-type Clostridium wechii. |
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| Keywords: | Escherichia coli C-type Clostridium wechii Inactivation Detoxification |
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