Field evaluation of antisense RNA mediated inhibition of GBSS gene expression in potato |
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| Authors: | G. J. Kuipers J. T. M. Vreem H. Meyer E. Jacobsen W. J. Feenstra R. G. F. Visser |
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| Affiliation: | (1) Dept. of Plant Breeding, Agricultural University Wageningen, P.O. Box 386, NL-6700 AJ Wageningen, The Netherlands;(2) Dept. of Genetics, University of Groningen, Kerklaan 30, NL-9751 NN Haren, The Netherlands |
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| Abstract: | Summary Granule-bound starch synthase (GBSS) catalyses the synthesis of amylose in starch granules. Analysis of antisense RNA mediated inhibition of GBSS gene expression in large numbers of tubers from in vitro grown, greenhouse grown and field grown transgenic potato plants revealed stable and total inhibition of GBSS gene expression in one clone. In three other transgenic genotypes partial and unstable inhibition was found. In these genotypes both GBSS activity and amylose content were remarkably reduced compared with the non-transformed control genotype. No relationship was found between the level of inhibition of GBSS gene expression and yield and dry matter content. |
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| Keywords: | Agrobacterium rhizogenes antisense RNA granule-bound starch synthase Solanum tuberosum starch composition transformation |
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