部分地方鸡种微卫星DNA指纹分析

利用20个微卫星标记对我国19个地方鸡种保种群进行了遗传检测,计算各群体的等位基因频率、平均基因杂合度、平均多态信息含量及各群体间的遗传距离,并用类平均法进行聚类分析。研究结果表明:20个微卫星标记在19个地方鸡种保种群共检测到184个等位基因,平均为9.2个,基因频率分布在0.013-0.838之间。19个地方鸡种平均杂合度在0.5824-0.7432之间。其中藏鸡最高,白耳鸡最低。平均多态信息含量在0.5238-0.7023之间,均大于0.5,表现为高度多态性;19个鸡种聚为6类。各鸡种的遗传距离及聚类结果与所保存的地方鸡种的地理分布、现实状况是相吻合的,从而表明用该方法分析品种间的亲缘关系是可行的。

Analysis on Microsatellite Fingerprinting in Native Chicken Breeds

The study analyzed the fingerprinting of 20 microsatellite loci with high polymorphism of 19 Native Chicken Breeds.The results showed that mean effective number of allele of 20 microsatellite loci in 19 Native Chicken Breeds was 9.2,the range of heterozygosity was 0.5824-0.7432,the heterozygosity of Zang chicken was the highest ( 0.7432),and that of white-ear chicken was the lowest (0.5824).the range of mean polymorphism information content was 0.5238-0.7023.which is higher than 0.5 and highly polymorphic.By the results of UPGMA tree,six groups were formed from 19 chicken breeds The results of microsatellite fingerprinting were in accordance with 19 populations'geographical distribution and actual status of 19 chickens.The results of cluster showed that microsatellite fingerpring was feasible to analysis genetic relationships among Chinese native chicken breeds.