| 二甲戊乐灵离体诱导酸枣八倍体的研究 |
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| 引用本文： | 路芳,代丽,刘孟军,赵锦.二甲戊乐灵离体诱导酸枣八倍体的研究[J].河北农业大学学报,2010,33(3). |
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| 作者姓名： | 路芳 代丽 刘孟军 赵锦 |
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| 作者单位： | 河北农业大学,中国枣研究中心,河北,保定,071001 |
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| 基金项目： | 河北省杰出青年基金项目，河北省科技支撑计划，国家科技支撑计划 |
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| 摘 要： | 以二甲戊乐灵为诱变剂,利用浸泡法对酸枣四倍体的茎段进行离体诱变,并采用流式细胞分析仪鉴定了形态发生变化的植株细胞染色体倍性。结果表明:二甲戊乐灵诱导酸枣八倍体的最适时间和浓度为200~300μmol/L的溶液处理8~10 d,诱变率为20.00%。在继代1次后,植株形态发生变化。继代4次后,经流式细胞仪测定为四倍体和八倍体的嵌合体。
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| 关 键 词： | 酸枣 二甲戊乐灵 八倍体 离体诱变 |
A study on octoploid induction of sour jujube (Ziziphus acidojujuba C. Y. Cheng et M. J. Liu) with pendimethalin in vitro |
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| LU Fang,DAI Li,LIU Meng-jun,ZHAO Jin.A study on octoploid induction of sour jujube (Ziziphus acidojujuba C. Y. Cheng et M. J. Liu) with pendimethalin in vitro[J].Journal of Agricultural University of Hebei,2010,33(3). |
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| Authors: | LU Fang DAI Li LIU Meng-jun ZHAO Jin |
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| Abstract: | Pendimethalin was used as mutagen,the shoot segments of the tetraploid plantlet of sour jujube were soaked in solution medium.Ploidy levels were determined by flow cytometry.The results showed that the optimum combination of concentration and time for octoploid induction of sour jujube was 200-300 μmol/L pendimethalin for 8-10 days.The doubling effect is 20.00%.The phenotypic variation was produced after one subculture.After four subcultures,chimeras of tetraploid and octoploid were identified by the flow cytometry. |
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| Keywords: | sour jujube(Ziziphus acidojujuba C Y Cheng et M J Liu) pendimethalin octoploid in vitro mutagenesis |
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