| 大孔树脂纯化人参茎叶提取液皂苷工艺研究 |
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| 引用本文： | 张丹,刘有良,李凤林.大孔树脂纯化人参茎叶提取液皂苷工艺研究[J].吉林农业科技学院学报,2014(1):16-18. |
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| 作者姓名： | 张丹 刘有良 李凤林 |
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| 作者单位： | [1]吉林农业科技学院生物工程学院,吉林132101 [2]吉林农业科技学院食品工程学院,吉林132101;吉林省酿造技术科技创新中心吉林132101 |
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| 基金项目： | 吉林农业科技学院大学生科技创新项目;吉林省教育厅“十二五”科学技术研究项目（吉教科合字2013第329号） |
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| 摘 要： | 对大孔树脂纯化人参茎叶提取液中皂苷工艺进行研究.选用D-101大孔吸附树脂,经预处理后装柱（层析柱）、确定吸附容量（皂苷∶干树脂重量=103∶1）、洗脱溶媒用量的确定（上柱液50ml,蒸馏水100mL,50％乙醇溶液100ml）、洗脱速度（1.5mL/min）,进行皂苷纯化.用高效液相色谱法进行含量测定.从测得数据可知,皂苷含量明显提高,说明D-101大孔吸附树脂纯化人参茎叶提取液总皂甙效果较佳.
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| 关 键 词： | 人参茎叶提取液 大孔树脂 纯化 皂甙 |
Purification of Saponin from Panax ginseng Stem and Leaf Extracts using Macroreticular Resin |
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| ZHANG Dan,LIU Youliang,LI Fenglin.Purification of Saponin from Panax ginseng Stem and Leaf Extracts using Macroreticular Resin[J].Journal Of Jilin Agricultural Science And Technology College,2014(1):16-18. |
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| Authors: | ZHANG Dan LIU Youliang LI Fenglin |
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| Institution: | 1. Jilin Agricultural Science and Technology University School of Bioengineering, Jilin 132101; 2. Jilin Agricultural Science and Technology University School of Food Engineering, Jilin 132101; 3. Jilin Province Innovation Center of Brewing Technology, Jilin 132101 ) |
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| Abstract: | The macroreticular resin purification of saponins in ginseng stem leaf extract technology research. Type of macroporous adsorbing resin：D-101,after the pretretment column packing （chromatographic column）to determine the elutive capacity（ saponins： the dry weight of resin--lO3：l）,to determine the volume of the eluant（the column liquid 5OraL,distilled water lOOmL 50% ethanol 100mL）,elution rate （1.SmUmin）, purification of saponins. Determination of sapoins by HPLC. From the measured data shows, saponin content significantly increased, D-IO1 macroporous adsorption resin purification of ginseng stem and leaf extract of total saponins is better. |
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| Keywords: | Panax ginseng leaf extracts macroreticular resin purification saponins |
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