巨大革耳遗传多样性的ISSR分析

为确定巨大革耳种质资源问的亲缘关系，本文应用ISSR分子标记技术，对来源不同地区的野生或栽培的9个巨大革耳菌株进行遗传多样性分析。从20个引物筛选获得4个ISSR多态性引物对巨大革耳菌株扩增，获得23条多态性条带，多态性比率为85．19％；对扩增结果进行聚类分析，当遗传距离为20％时，9个菌株聚为2类：I类包括C．m0002菌株；Ⅱ类包括其它的8个菌株。其中C．m0002菌株与其它8个菌株的遗传距离很远。经栽培出菇实验，结果表明，C．m0002菌株的子实体似多脂鳞伞（黄伞），是同名异种；其它8个菌株均为巨大革耳。ISSR分析的结果与子实体形态特征一致。

Analysis of Genetic Diversity among Germplasm of Panus giganteus by ISSR Markers

In order to evaluate the genetic relationship among germplasm of Panus giganteus, the genetic diversity of 9 P. giganteus strains from different regions of china was analyzed using ISSR markers. 4 ISSR primers were selected from 20 primers were applied to amplify the DNA of 9 strains. The results showed that polymorphic bands were 23, and the poly- morphic rates were 85.19%. UPDMA dendrogram based on ISSR markers indicated that 9 P. giganteus strains could be distinguished into 2 groups when genetic distance was 20%, and the I group included C.m0002 strain, the group II in- cluded the other 8 strains. The genetic distance between C.m0002 strain and the other 8 strains was large. The strain C. m0002 might be Phonliota adipose, and the other 8 strains were Panus giganteus by cultivation experiment. Their genetic relation based on ISSR markers was corresponded with the one based on fruit body traits.