| 微管相关蛋白2微管结合区基因的克隆与表达 |
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| 引用本文： | 郭 燕,张彦明,高 晨.微管相关蛋白2微管结合区基因的克隆与表达[J].西北农林科技大学学报(社会科学版),2006,34(8):7-11. |
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| 作者姓名： | 郭 燕 张彦明 高 晨 |
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| 作者单位： | 1. 西北农林科技大学,动物科技学院,陕西,杨凌,712100
2. 中国疾病预防控制中心,病毒病预防控制所,北京,100052 |
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| 摘 要： | 为了研究微管相关蛋白2(M AP 2)在促进微管形成、维持微管稳定及促进轴突和树突细胞器转运等方面的作用,试验克隆了M AP 2微管结合区基因,构建了原核表达载体pET 32a-M AP 2并对其进行诱导表达,利用亲和层析法对表达产物进行纯化,研究了表达产物与微管蛋白之间的作用。结果表明,融合表达的人M AP 2微管结合区蛋白约43 ku,可与天然的微管蛋白发生分子间的结合作用,为M AP 2生理功能的研究奠定了基础。
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| 关 键 词： | MAP2 表达纯化 微管蛋白 |
| 文章编号： | 1671-9387（2006）08-0007-05 |
| 收稿时间： | 2006-02-20 |
| 修稿时间： | 2006-02-20 |
Expression and purification of human MAP2 microtubule binding peptide |
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| GUO Yan,ZHANG Yan-ming,GAO Chen,HAN Jun,CHEN Jian-ming,SHI Qi,GAO Yong-jun,ZHOU Wei,DONG Xiao-ping.Expression and purification of human MAP2 microtubule binding peptide[J].Journal of Northwest Sci-Tech Univ of Agr and,2006,34(8):7-11. |
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| Authors: | GUO Yan ZHANG Yan-ming GAO Chen HAN Jun CHEN Jian-ming SHI Qi GAO Yong-jun ZHOU Wei DONG Xiao-ping |
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| Institution: | 1 College of Animal Science and Technology,Northwest A & F University,Yangling,Shaanxi 712100,China;2 Chinese Center for Viral Disease Control and Prevention,Beijing 100052,China) |
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| Abstract: | In order to study the effects of MAP2 on the formation and stabilization of microtubule as well as the potential transportation functions in axon and synapse,the gene encoding microtubule binding region of MAP2 was cloned and expressed. Verified by sequence analysis and enzyme digested,an recombinant vector pET32a-MAP2 was constructed. A 43 ku soluble MAP2-fusion protein was expressed in E. coli BL21 and purified by affinity chromatography. The interaction of the proteins was studied and it was suggested that MAP2 microtubule binding peptide could interact with each other in vitro. It can provid a good basis for further research on the biological functions of MAP2. |
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| Keywords: | MAP2 |
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