| 一种高效建立小麦再生系统的方法 |
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| 引用本文： | 伍玲,朱华忠. 一种高效建立小麦再生系统的方法[J]. 西南农业学报, 2007, 20(2): 262-265 |
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| 作者姓名： | 伍玲 朱华忠 |
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| 作者单位： | 四川省农业科学院作物研究所,四川,成都,610066 |
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| 基金项目： | 人事厅留学回国人员资助项目;四川省青年基金项目（05ZQ026-0050） 致谢：本实验完成于四川省农科院生核所生物技术中心,特此感谢. |
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| 摘 要： | 本文介绍了一种从CIMMYT学到的小麦幼胚培养方法,并研究了MS培养基和源于CIMMYT的MSE3培养基在四川18份小麦品种的幼胚培养上的差异。结果显示①胚性愈伤组织诱导率在MSE3培养基中比在MS培养基中平均高18.2%,表明MSE3培养基能明显提高胚性愈伤组织的诱导率;②四川小麦品种绵农7号在MSE3诱导培养基中和分化培养基中,其出愈率、胚性愈伤组织诱导率、绿原基分化率和出苗率全部达到了100%。表明该品种具有优异胚性化能力。
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| 关 键 词： | 小麦 再生系统 幼胚培养方法 |
| 文章编号： | 1001-4829（2007）02-0262-04 |
| 修稿时间： | 2007-01-12 |
One effective method to built regeneration system of wheat |
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| WU Ling,ZHU Hua-zhong. One effective method to built regeneration system of wheat[J]. Southwest China Journal of Agricultural Sciences, 2007, 20(2): 262-265 |
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| Authors: | WU Ling ZHU Hua-zhong |
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| Affiliation: | Crop Research Institute, Siehuan Academy of Agricultural Sciences, Sichuan Chengdu 610066, China |
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| Abstract: | The method of immature embryo culture used in CIMMYT was employed to evaluate the ability of immature embryo culture in 18 Sichuan wheat cultivars by MS medium and MES3 medium(from CIMMYT).The results indicated that:(i) the frequency of embrogenic callus induction in MSE3 medium was higher 18.2 % than that of in MS medium,it is seemed that MES3 medium could improve the frequency of embrogenic callus induction;(ii) one Sichuan wheat cultivar MN7 with high regeneration ability of immature embryo culture,was selected. |
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| Keywords: | wheat regeneration system method of immature embryo culture |
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