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蓝舌病病毒分离株的定型研究
引用本文:马洪超,杨承谕,普淑英,陈兴扶,李晓成,范根成,张燕霞.蓝舌病病毒分离株的定型研究[J].中国动物检疫,1993(5):9-11.
作者姓名:马洪超  杨承谕  普淑英  陈兴扶  李晓成  范根成  张燕霞
作者单位:农业部动物检疫所 青岛266032 (马洪超,杨承谕,普淑英,陈兴扶,李晓成,范根成),农业部动物检疫所 青岛266032(张燕霞)
摘    要:用国际标准蓝舌病病毒(BTV)型特异性血清和新制备的BTV型特异性血清,按OIE推荐方法进行蚀斑抑制试验,并试用“悬浮法”蚀斑抑制试验,对BTV分离株(L001)作了血清型鉴定,结果表明该分离株为BTV16型。与标准毒株相对照,两者试验结果完全一致;L001株的RNA的PAGE电泳带谱与BTV16型标准株的带谱相同。

关 键 词:蓝舌病病毒  蚀斑抑制试验  病毒定型

Identification and Serotyping of a Bluetongue Virus Isolate with Plaque Inhibition Test
Ma Hong-chao,Yang Cheng-yu,Pu Shu-ying,Chen Xing-fu,Li Xiao-cheng,Fan Gen-cheng and Zhang Yan-xia.Identification and Serotyping of a Bluetongue Virus Isolate with Plaque Inhibition Test[J].China Journal Of Animal Quarantine,1993(5):9-11.
Authors:Ma Hong-chao  Yang Cheng-yu  Pu Shu-ying  Chen Xing-fu  Li Xiao-cheng  Fan Gen-cheng and Zhang Yan-xia
Abstract:A BTV isolate (L001) was serotyped against international standard BTV-type specific sera and the laboratory prepared BTV type-specific sera using both OIE recommended plaque inhibition test and the modified plaque inhibition test (suspension plaque inhibition test). The results showed that L001 isolate was identical to the standard strain of BTV-16 in both plaque inhibition tests and PAGE pattern of RNA from L001 was the same as that of the standard strain of BTV-16. L001 isolate is believed to belong to BTV-16.
Keywords:bluetongue virus  Serotyping  Plaque Inhibition Test  
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