| 低温胁迫下小麦生理生化及蛋白组分变化研究 |
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| 引用本文： | 左芳,范宝莉. 低温胁迫下小麦生理生化及蛋白组分变化研究[J]. 安徽农业科学, 2009, 37(4): 1430-1431 |
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| 作者姓名： | 左芳 范宝莉 |
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| 作者单位： | 天津师范大学化学与生命科学学院,细胞遗传与分子调控天津市重点实验室,天津,300387;天津师范大学化学与生命科学学院,细胞遗传与分子调控天津市重点实验室,天津,300387 |
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| 摘 要： | [目的]探究间接冷害对小麦幼苗生长的影响和影响机制。[方法]用水培法培养冬小麦京411，对实验组幼苗实施4℃低温胁迫。测定幼苗长势，丙二醛含量以及可溶性蛋白含量变化，并应用垂直板聚丙烯酰胺凝胶电泳对幼苗叶片内的蛋白组分进行分离，研究低温胁迫对小麦幼苗生理生化及蛋白组分的影响。[结果]结果表明，低温胁迫下，小麦幼苗叶片出现卷曲和萎蔫，叶片丙二醛含量、叶片内的蛋白组分含量和可溶性蛋白含量均有明显下降。[结论]该研究为探讨低温胁迫机理提供理论依据。
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| 关 键 词： | 低温胁迫 小麦 丙二醛 可溶性蛋白 聚丙烯酰胺凝胶电泳 |
Research on the Physiological-Biochemical and Protein Component Changes of Wheat under Low Temperature Stress |
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| ZUO Fang et al. Research on the Physiological-Biochemical and Protein Component Changes of Wheat under Low Temperature Stress[J]. Journal of Anhui Agricultural Sciences, 2009, 37(4): 1430-1431 |
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| Authors: | ZUO Fang et al |
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| Affiliation: | ZUO Fang et al(Tianjin Key Laboratory of Cyto-Genetical , Molecular Regulation,College of Chemistry , Life Science,Tianjin Normal University,Tianjin 300387) |
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| Abstract: | [Objective]The purpose was to explore the effects of indirect chilling injury on the growth of wheat seedlings and the mechanism of the influence.[Method]The water culture was used to cultivate winter wheat Jing 411,and then study the growth of the seedlings,survey the content changes of MDA and dissoluble proteins.The vertical SDS-PAGE was used to separate the proteins from the leaves and study the effect of low temperature stress on the physiological-biochemical and protein component changes.[Result]The r... |
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| Keywords: | Low temperature stress Wheat MDA Dissoluble protein SDS-PAGE |
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