中国松树枯梢病菌营养体亲和性研究

将来自我国13省16种松树和其它2种针叶树上的55个Sphaeropsis sapinea菌株在PDA 平皿上进行配对生长试验,结果绝大多数菌株间产生墨绿色色素隔离带,少数菌株以及同一菌株不同菌落间不产生此色素带。显微观察表明,在产生色素带的菌株间未发现菌丝融合,而在不产生色素带的菌株间可见到菌丝融合。测定S.sapinea 营养体亲和与否可通过观察有无形成色素反应带来确定。55个供试菌株共可分为48个VCG_S,其中44个VCG_S各只含一个菌株,3个VCG_S分别含2个菌株,只有一个VCG_S含5个菌株。

STUDY ON VEGETATIVE COMPATIBILITY OF SPHAEROPSIS SAPINEA IN CHINA

Fifty|five isolates of %S.sapinea% were obtained on 16 pines and other 2 conifers from 13 Provinces in China. Mycelial parings of the isolates on PDA produced either an incompatible reaction (most of isolates) in which a reaction color line (dark green) between the two isolates developed in interaction zone, or a compatible reaction (few isolates and same isolates) in which no reaction color line developed. Microscopical observation showed that hyphal anastomosis was not found on the former (with reaction line), but found on the latter (no reaction line). So vegetative compatibility of %S.sapinea% could be measured by observing development of reaction color line between two isolates.;Among the 55 isolates, 48 vegetative compatibility groups (VCG-S) were recognized, and each of 44 VCG-S included only 1 isolate, 3 VCG-S included 2 isolates, respectively, and 1 VCG included 5 isolates.