| 宫内发育迟缓仔猪小肠形态和屏障功能相关基因的表达特征 |
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| 引用本文： | 唐武,张旺宏,孔祥峰.宫内发育迟缓仔猪小肠形态和屏障功能相关基因的表达特征[J].动物营养学报,2022,34(2):1240-1246. |
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| 作者姓名： | 唐武 张旺宏 孔祥峰 |
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| 作者单位： | 中国科学院亚热带农业生态研究所,动物营养生理与代谢过程湖南省重点实验室,长沙 410125;中国科学院大学,北京 100049 |
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| 基金项目： | 国家自然科学基金面上项目(31772613);湖南省科技领军人才项目(2019RS3022)。 |
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| 摘 要： | 为了研究宫内发育迟缓(IUGR)仔猪小肠形态和屏障功能相关基因的表达特征,选取24窝"长白×大白"杂交新生仔猪,每窝选取1头IUGR仔猪和1头正常出生体重(NBW)仔猪,分别于7、21和28日龄屠宰8头IUGR仔猪和8头NBW仔猪,采集小肠样品进行分析.结果表明:1)与NBW仔猪相比,IUGR仔猪7日龄时空肠绒毛高度、...
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| 关 键 词： | 宫内发育迟缓 仔猪 肠道形态 紧密连接蛋白 免疫细胞因子 |
Profiles of Small Intestinal Morphology and Gene Expression Related to Intestinal Barrier Function in Piglets with Intrauterine Growth Retardation |
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| TANG Wu,ZHANG Wanghong,KONG Xiangfeng.Profiles of Small Intestinal Morphology and Gene Expression Related to Intestinal Barrier Function in Piglets with Intrauterine Growth Retardation[J].Acta Zoonutrimenta Sinica,2022,34(2):1240-1246. |
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| Authors: | TANG Wu ZHANG Wanghong KONG Xiangfeng |
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| Institution: | (Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;University of Chinese Academy of Sciences,Beijing 100049,China) |
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| Abstract: | The present study was conducted to determine the profiles of small intestinal morphology and gene expression related to intestinal barrier function in piglets with intrauterine growth retardation. Twenty-four litters of Landrace×Large White neonatal piglets were used in this study,and one IUGR piglet and one normal birth weight(NBW)piglet were selected from each litter. Eight IUGR piglets and eight NBW piglets were selected and slaughtered at 7,21 and 28 days of age,respectively,for small intestine sample collection and analysis.The results showed as follows:1)compared with the NBW piglets,the villus height in jejunum of IUGR piglets at 7 days of age and the villus height in jejunum and ileum at 21 days of age were significantly decreased(P<0.05),while the crypt depth in jejunum at 28 days of age was significantly increased(P < 0.05). 2)Compared with the NBW piglets,the mRNA relative expression levels of zonula occluden-1(ZO-1),claudin-1,occludin,interleukin-4(IL-4)and interleukin-8 in jejunal mucosa of IUGR piglets at 7 days of age and the mRNA relative expression levels of ZO-1 and occludin in jejunal mucosa and ZO-1 in ileal mucosa at21 days of age were significantly down-regulated(P<0.05);the mRNA relative expression levels of tumor necrosis factor-αin jejunal mucosa and interleukin-1βin ileal mucosa at 28 days of age were significantly upregulated(P<0.05),while the mRNA relative expression level of IL-4 in ileal mucosa was significantly downregulated(P<0.05). In summary,in IUGR piglets,jejunum and ileum morphology is impaired,tight junction protein gene expression is down-regulated,and immune cytokines expression is abnormal,which further affects intestinal barrier function.Chinese Journal of Animal Nutrition,2022,34(2):1240-1246] |
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| Keywords: | intrauterine growth retardation piglets intestinal morphology tight junction protein immune cytokine |
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