Production of desert locust feeding deterrents from in vitro cultured neem (Azadirachta indica) |
| |
Authors: | Silvia Zypman Meira Ziv Shalom W Applebaum |
| |
Institution: | (1) Dept. of Agricultural Botany and the Otto Warburg Center for Agricultural Biotechnology, USA;(2) Dept. of Entomology, The Hebrew University of Jerusalem, Faculty of Agricultural, Food and Environmental Quality Sciences, 76100 Rehovot, Israel |
| |
Abstract: | Callus was initiated from cotyledon, hypocotyl and shoot tip explants of neem seedlings (Azadirachta indica A. Juss.) that
were cultured on Murashige and Skoog (MS) agar medium supplemented with MS vitamins, casein hydrolysate, indole-3-acetic acid
and benzylamino purine. Shoots regenerated from hypocotyl-derived callus only. Mature 10-12-week-old callus regenerated both
shoots and somatic embryos (SEm). The antifeedant activity of different types of callus, leaves and SEm was determined with
larvae of the desert locust Schistocerca gregaria in a no-choice feeding bioassay. In vitro tissue-cultured neem had antifeedant
properties against the desert locust. To the best of our knowledge the antifeedant activity of neem somatic embryos extracts
is reported here for the first time. |
| |
Keywords: | Azadirachta indica neem tissue culture Schistocerca gregaria desert locust antifeedants no-choice feeding bioassay |
本文献已被 SpringerLink 等数据库收录! |