| H5N1亚型高致病性禽流感病毒A/duck/HuBei/49/05株反向基因操作系统的建立 |
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| 引用本文： | 苏胜杰,许静,宋家升,李雁冰,希尼尼根.H5N1亚型高致病性禽流感病毒A/duck/HuBei/49/05株反向基因操作系统的建立[J].畜牧与饲料科学,2010,31(2):20-21,27. |
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| 作者姓名： | 苏胜杰 许静 宋家升 李雁冰 希尼尼根 |
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| 作者单位： | 1. 内蒙古农业大学兽医学院,内蒙古,呼和浩特,010018
2. 中国农业科学院哈尔滨兽医研究所,农业部动物流感重点开放实验室,兽医生物技术国家重点实验室,黑龙江,哈尔滨,150001 |
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| 摘 要： | 水禽是禽流感病毒的天然储存库,为了研究高致病性禽流感病毒对水禽致病性的分子基础,研究构建了DKHB/49/05的8质粒反向遗传操作系统。用Trizol从含A/duck/HuBei/49/05株病毒的鸡胚尿囊液中提取总RNA,用RT-PCR扩增PB2、PB1、PA、HA、NP、NA、M、NS基因片段,将其分别克隆至pBD载体中。利用8质粒反向遗传操作系统,将重组的pBD质粒共转染293T细胞,成功拯救了该病毒,并命名为R-DKHB。
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| 关 键 词： | H5N1 禽流感病毒 反向遗传 |
Establishment of Highly Pathogenic H5N1 Avian Influenza Virus A/duck/HuBei/49/05 Base on Reverse Genetics System |
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| SU Sheng-jie,XU Jing,SONG Jia-sheng,LI Yan-bing,Xininigen.Establishment of Highly Pathogenic H5N1 Avian Influenza Virus A/duck/HuBei/49/05 Base on Reverse Genetics System[J].Animal Husbandry and Feed Science,2010,31(2):20-21,27. |
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| Authors: | SU Sheng-jie XU Jing SONG Jia-sheng LI Yan-bing Xininigen |
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| Institution: | 1.College of Veterinary Science/a>;Inner Mongolia Agricultural University/a>;Hohhot 010018/a>;China/a>;2.Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology/a>;Harbin Veterinary Research Institute CAAS/a>;Harbin 150001/a>;China |
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| Abstract: | Waterfowl are the natural repository of avian influenza virus.To study the pathogenic molecular basis of highly pathogenic avian influenza from waterfow,8 recombinant PBD plasmids and rescued the recombinant virus were constructrd by reverse genetics techniques.The total RNA was extracted from the allantoic fluids of chick embryo with the infection of A/duck/HuBei/49/05 virus,PB2,PB1,PA,HA,NP,NA,M and NS were amplified by RT-PCR respectively,and they were cloned into PBD vector.Moreover,they also were trans... |
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| Keywords: | H5N1 avian influenza virus reverse genetics |
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