兔体内纤维素酶的分离及酶学性质的研究

目的]研究兔消化系统中内源性纤维素酶及其酶学性质。方法]取兔子消化系统中的胃、十二指肠、小肠和大肠,用DNS法测定纤维素酶活并对其性质进行研究。结果]结果表明,胃、十二指肠、小肠和大肠纤维素酶活性最高的底物浓度均是0.625%,最适显色时间5 min,最适pH值分别是4、6、7、6,最适温度是48℃。结论]该研究结果为兔饲料及其应用研究提供一定的理论基础。

Studies on Separation and Enzymatic Character of Cellulase from Rabbit

Objective]The purpose of this research was to study the endogenous cellulose and its enzymatic character in digestive system of rabbit.Method] The stomach,the duodenum,the small intestine and the large intestine in digestive system of rabbit were taken to study its zymology activeness.DNS menthod was used to analyze the effects of determination conditions on results of measurements.Result]The results indicated that under the different conditions,bodies cellulase activities were different.The stomach,the duodenum,the small intestine,the large intestine most suitable substrate concentration were 0.625%,the most suitable colored time was 5 min,most suitable pH value was 4,6,7 and 6,respectively.The stomach,the duodenum intestines,the small intestine and the large intestine optimum temperature was 48 ℃.Conclusion]This research result will provide the theoretical references for rabbit foodstuff and application of additive.