盐胁迫对转BADH基因水稻R1的影响

以转BADH基因水稻R1和常规对照品种为材料,采用3种不同浓度NaCl在苗期和孕穗期进行盐胁迫处理,分析了R1和对照之间的株高、BADH活性、叶片相对电导率和植物大分子渗漏值等指标,并进行PCR分子检测。结果表明,PCR阳性与阴性植株个数分离比不完全符合简单的孟德尔遗传规律;相对电导率和植物大分子渗漏值低,而BADH活性高的转基因植株耐盐性比对照增强。此结果说明转基因水稻R1耐盐性明显高于对照,目的基因正常表达,此结果对今后培育新的抗性品种奠定了基础。

Effect of Salt Stress on R1 Generation of Transgenic Rice

Four R1 lines of transgenic rice and check variety "Fengyou 516" were treated under different salt stress.Four indexes and PCR detection were determined and analyzed.The results showed that 1) the ratio of negative numbers to positive ones of PCR detection was not completely conform to the first Mendelian law;2) The plant height of transgenic R1 generation was higher than non-transgenic plants under salt stress;3) The activity of BADH of transgenic R1 plants increased greatly while that of non-transgenic plants was undetectable and 4) Relative electronic conductivity and leakage rate of macromolecules of transgenic R1 plants was lower than non-transgenic plants.This indicated that the BADH gene already expressed in the transgenic R1 generation.These results can contribute to the rice breeding in the future.