斑痣悬茧蜂和斜纹夜蛾核多角体病毒的互作及氟啶脲对寄生蜂的影响

为探寻小同生物防治资源协同控制害虫的可行性,研究了斑痣悬茧蜂和斜纹夜蛾核多角体病毒(SIN-PV)联合作用于斜纹夜蛾时两者的相互影响,以及昆虫生长调节剂氟啶脲对斑痣悬茧蜂的影响,结果表明,在SIN-PV浓度为10~6PIB/ml、10~7PIB/ml和10~8 PIB/ml 3个浓度下,无沦是病毒和寄生蜂一同处理,还是先寄牛蜂处理1 d后冉病毒处理,斑痣悬茧蜂对SINPV的侵染力和侵染速度均无显著影响.联合处理时病毒埘寄牛蜂也无不良影响,蜂后代结茧数与对照无差异,仅缩短了先寄生蜂后10~6PJB/mJ SINPV处理组寄生蜂从卵刮茧的发育历期.从氟啶脲对斑痣悬茧蜂的影响来看,寄生蜂寄生经1.25 mg/L氟啶脲处理后的甜菜夜蛾,其后代发育历期和结茧均未受影响,仅羽化受影响.寄生蜂成虫卣接经10 ms/L、20 mg/L和40 mg/L(田间推荐剂量)氟啶脲处理后,均对后代结茧和发育历期等无显著影响.表明斑痣悬茧蜂可较安全地与一定浓度范围斜纹夜蛾核多角体病毒或氟啶脲联合用于夜蛾类害虫的控制.

Interaction of Meteorus pulchriconis with Spodoptera litura Neuclopolyhedrovirus and Impact of Chlorfluazuron on Parasitoid

In order to evaluate the feasibility of combination of different biological agents to control insect pests, the interaction of Meteorus pulchriconis with Spodoptera litura neuclopolyhedrovirus (SINPV) were studied, and the impact of chlorfluazuron, an insect developmental regulator, on Meteorus pulchriconis was also investigated. The results showed that the biological activities and killing speeds of 10~6 PIB/ml SINPV , 10~7 PIB/ml SINPV and 10~8 PIB/ml SINPV combined with Meteorus pulchriconis were not significantly different from those of SINPV alone. And there was also no negative effect of SI-NPV on pupation of Meteorus pulchriconis, except that duration from egg to pupation was shortened when Spodoptera litura larvae were parasitized for 1d and then infected by 10~6 PIB/ml SINPV. It was also found that chlorfluazuron only impacted the emergence of Meteorus pulchriconis when they parasitized S. exigua treated with 1. 25 mg/L chlorfluazuron. As the para-sitoid was treated with 10 rag/L, 20 mg/L and 40 mg/L chlorfluazuron in adult stage, pupation and developmental duration of their offsprings were not affected. The resultsindicated that Meteorus pulchriconis could be combined with SINPV or chlorfluazuron to control noctuid moths safely.