Isolation and characterization of the heat stable enterotoxin from a pathogenic bovine strain of Escherichia coli |
| |
| Authors: | C Gerday M Herman J Olivy N Gerardin-Otthiers D Art E Jacquemin A Kaeckenbeeck J Van Beeumen |
| |
| Affiliation: | 1. Laboratoire de Biochimie musculaire, Université de Liège, Institut de Chimie, Sart Tilman, 4000 Liège Belgium;2. Laboratoire de Pathologie des maladies bactériennes, Université de Liège, Faculté de Médecine Vétérinaire, 45, rue des Vétérinaires, 1070 Bruxelles Belgium;3. Laboratorium voor Microbiologie en microbiele Genetica, Faculteit Wetenschappen, Rijks Universiteit Gent, K.L. Ledeganckstraat, 35, 9000 Gent Belgium |
| |
| Abstract: | A heat-stable enterotoxin secreted by a pathogenic strain of Escherichia coli of calf origin was purified to homogeneity by a procedure involving acetone fractionation, DEAE cellulose chromatography, Biogel P2 chromatography and size exclusion high pressure liquid chromatography. The purity of the product was ascertained by amino-acid analyses and amino acid sequence using manual degradation with 4-N, N dimethylaminoazobenzene-4' isothiocyanate (DABITC) and an automatic gas phase sequenator. The following amino acid sequence is proposed: Asn-Thr-Phe-Tyr-Cys-Cys-Glu-Leu-Cys-Cys-Asn-Pro-Ala-Cys-Ala-Gly-Cys-Tyr. It is identical to a similar active peptide isolated from strains of porcine origin. Antibodies to ST were successfully produced in rabbits using a conjugate with bovine serum albumin. The ultraviolet absorption and circular dichroism spectra of the active product were recorded and discussed. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
