| 磺胺二甲基嘧啶人工抗原合成及多克隆抗体制备 |
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| 引用本文： | 张伟,钟理,许若丹,赵美玲,吴策. 磺胺二甲基嘧啶人工抗原合成及多克隆抗体制备[J]. 河北农业大学学报, 2005, 28(6): 76-80 |
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| 作者姓名： | 张伟 钟理 许若丹 赵美玲 吴策 |
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| 作者单位： | 河北大学,生命科学学院,河北,保定,071002;河北大学,生命科学学院,河北,保定,071002;University of Kentucky College of Medicine, Lexington, KY 40536, USA |
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| 摘 要： | 磺胺二甲基嘧啶(SM2)为只具有反应原性而无免疫原性的半抗原。本试验将SM2重氮化后分别连接人血清白蛋白(HSA)、卵清蛋白(OVA),合成人工抗原,经紫外光谱扫描验证偶联结果,计算结合比分别为4∶1、3∶1。用偶联物SM2-HSA免疫新西兰白兔,以SM2-OVA为包被原,4次免疫后ELISA方法测定抗血清效价为1∶12 800。表明抗原合成成功,这为小分子药物残留的免疫学检测奠定了技术基础。
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| 关 键 词： | 磺胺二甲基嘧啶 人工抗原 ELISA 多克隆抗体 |
| 文章编号： | 1000-1573（2005）06-0076-05 |
| 收稿时间： | 2005-06-28 |
| 修稿时间： | 2005-06-28 |
Preparation of artificial antigen and polyclonal antibody for sulfamethazine immunoassay |
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| ZHANG Wei,ZHONG Li,XU Ruo-dan,ZHAO Mei-ling,WU Ce. Preparation of artificial antigen and polyclonal antibody for sulfamethazine immunoassay[J]. Journal of Agricultural University of Hebei, 2005, 28(6): 76-80 |
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| Authors: | ZHANG Wei ZHONG Li XU Ruo-dan ZHAO Mei-ling WU Ce |
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| Affiliation: | 1. College of Life Science, Hebei University, Baoding 071002, China; 2. University of Kentucky College of Medicine, Lexington, KY 40536, USA |
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| Abstract: | Sulfamethazine(SM_2) is a small molecular hapten possessing immunoreactivity but not possessing immunogenicity.It was coupled with human serum albumin(HSA) and ovalbumin(OVA) by diazotization.The complete antigens were identified by UV scan,and the molecular ratio of SM_2 to HSA and OVA were 4 and 3.The conjugate of SM_2-HSA was used to immunize rabbits.After 4 times of booster injection,the produced antiserum showed special sensitivity and high titer to the Sulfamethazine in the indirect ELISA.The results demonstrate that the conjugate of SM_2-HSA possess immunogenicity,which provides a basis for immunoassay of hapten-drug residue. |
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| Keywords: | sulfamethazine artificial antigen ELISA polyclonal antibody |
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