抗戊唑醇禾谷丝核菌菌株对不同碳氮营养源的利用

用菌落直径法和菌丝干重法研究了查彼克（Czapek）培养基中葡萄糖、果糖、半乳糖、木糖、甘油5种碳源和硫酸铵、硝酸钠、亚硝酸钠、尿素、丙氨酸、亮氨酸、丝氨酸、脯氨酸、精氨酸、组氨酸、胱氨酸、赖氨酸12种氮源对抗戊唑醇禾谷丝核菌Rhizoctonia cerealis菌株生长的影响,以探明抗性菌株和敏感菌株之间生理适合度的差异.结果表明,在不同碳源条件下,所有菌株都在蔗糖、甘油和葡萄糖中生长最快,培养7天后,菌落直径和菌丝干重分别可达3.1750～7.5667 cm和0.0334～0.0554g,在木糖和果糖中生长相对较慢;在相同碳源条件下,高抗菌株（WWL）的生长速度小于敏感菌株（WW）和中抗菌株（WX1和WX2）,敏感菌株和中抗菌株之间的生长速度差异不明显.在不同氮源条件下,所试菌株均能较好利用硝酸盐、亚硝酸盐、精氨酸和丙氨酸,菌落直径和菌丝干重分别可达6.2～8.8 cm和0.0336～0.0543 g,不能充分利用胱氨酸,菌落直径和菌丝干重分别仅为1.4217～3.4750 cm和0.0178～0.0297 g;在同一氮源条件下,高抗菌株的生长速度小于敏感菌株和中抗菌株,而敏感菌株和中抗菌株之间差异不明显.

Utilization of different carbon and nitrogen nutrition sources to tebuconazole-resistant strains of Rhizoctonia cerealis

In order to study the changes of physiological fitness between the tebuconazole-resistant strains and tebuconazole-sensitive strains of Rhizoctonia cerealis, different carbon source and nitrogen source were selected to prepare Czapak medium and to study their effect on the colony diameter and dry weight. The carbon source included dextrose, sucrose, fructose, galactose, xylose, glycerin respectively, and the nitrogen source included ammonium sulfate, sodium nitrate, sub-sodium nitrate, urea, alanine, leucine, serine, proline, arginine, histidine, cysteine, lysine respectively. The results showed that, in the different carbon source all the strains grew fastest in dextrose, glycerin and sucrose after cultured 7 days, the colony diameter and dry weight were 3.1750-7.5667cm and 0.0334-0.0554g respectively, but grew slowest in xylose and fructose. In the same carbon source, high tebuconazole-resistant strain (WWL) grew slower than tebuconazole-sensitive strain (WW) and middling tebuconazole-resistant strains (WW1 and WW2), but no any obvious difference among WW, WW1 and WW2. In the different nitrogen sources, all the strains could utilize nitrites, sub-nitrates, arginine and ammonia more effectively, the colony diameter and dry weight were 6.2-8.8cm and 0.0336-0.0543g respectively, but could not utilize cysteine, the colony diameter and dry weight were only 1.4217-3.4750cm and 0.0178-0.0297g respectively. In the same nitrogen source, WWL grew slower than WW, WW1 and WW2, however, the difference of growth speeds of WW, WW1 and WW2 was not marked.